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http://dx.doi.org/10.5010/JPB.2022.49.2.155

Antioxidant and anti-inflammatory effects of seed ethanol extracts of Rubus coreanus miquel  

Hwang, Jin-Woo (Department of Medical Laboratory Science, College of Health Science, Dankook University)
Kang, Hyun (Department of Medical Laboratory Science, College of Health Science, Dankook University)
Lee, Sung-Gyu (Department of Medical Laboratory Science, College of Health Science, Dankook University)
Publication Information
Journal of Plant Biotechnology / v.49, no.2, 2022 , pp. 155-161 More about this Journal
Abstract
This study investigated the antioxidant and anti-inflammatory effects of seed ethanol extracts from Rubus coreanus Miquel (SERC). To investigate the antioxidant activity, total polyphenol and flavonoid content, ABTS and DPPH radical scavenging activity, and reducing power were measured. The total polyphenol and flavonoid contents in seed ethanol extracts of R. coreanus Miq. were 4.09 ㎍ gallic acid equivalents (GAE)/mg and 16.25 ㎍ quercetin equivalents (QE)/mg, respectively. DPPH and ABTS radical scavenging activity showed concentration-dependent scavenging activity, and the RC50 values of SERC were 26.68 ㎍/mL and 39.30 ㎍/mL, respectively. Moreover, the ferric reducing antioxidant power (FRAP) assay was performed to assess the reducing power, and SERC showed 0.61 ± 0.01 mM FeSO4 E/mg. To measure the anti-inflammatory effect, the cytotoxicity and nitric oxide (NO) production inhibitory efficacy in RAW 264.7 cells was confirmed. SERC showed a NO production inhibitory effect at 500 ㎍/mL without cytotoxicity. As a result of verifying the antioxidant and anti-inflammatory activity using SERC, its potential as an antioxidant and anti-inflammatory material was confirmed.
Keywords
Seed of Rubus coreanus Miq.; Ethanol extracts; Antioxidant; Anti-inflammatory; Cytotoxicity;
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Times Cited By KSCI : 8  (Citation Analysis)
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