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http://dx.doi.org/10.5010/JPB.2020.47.3.227

Production of miraculin protein in suspension cell lines of transgenic rice using Agrobacterium  

Kim, Hee Kyoung (Division of Horticultural Biotechnology, Hankyong National University)
Go, Ji Yun (Division of Horticultural Biotechnology, Hankyong National University)
Park, So-Young (Department of Horticultural Science, Division of Animal, Horticultural and Food Sciences, Chungbuk National University)
Kang, Kwon Kyoo (Division of Horticultural Biotechnology, Hankyong National University)
Jung, Yu Jin (Division of Horticultural Biotechnology, Hankyong National University)
Publication Information
Journal of Plant Biotechnology / v.47, no.3, 2020 , pp. 227-234 More about this Journal
Abstract
To produce the miraculin protein in suspension cultures, rice (Oryza sativa L.) was transformed with Agrobacterium tumefacience EHA105 containing the miraculin AB512278 gene. The cell suspension cultures were established using cell lines selected from transgenic rice callus. The integration of the miraculin gene into the rice chromosome was confirmed using genomic PCR analysis. In addition, RT-PCR analysis indicated that the miraculin gene is expressed in the selected suspension cell lines. Thus, the recombinant miraculin was expressed in the transgenic suspension cell line, HK-2. Therefore, we have successfully developed a HK-2 line that produces miraculin. These results demonstrate that transformed cell suspension cultures can be used to produce a taste-modifying protein such as miraculin.
Keywords
Cell suspension culture; Rice transformation; Miraculin; Taste-modifying protein;
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Times Cited By KSCI : 6  (Citation Analysis)
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