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http://dx.doi.org/10.5010/JPB.2018.45.4.364

Development of transgenic potato with improved anthocyanin contents using sweet potato IbMYB1 gene  

Kim, Yun-Hee (Department of Biology Education, College of Education, IALS, Gyeongsang National University)
Han, Eun-Hee (Medicinal Recourses Research Institute, Gyeongnam Agricultural Research & Extension Services)
Kwak, Sang-Soo (Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Lee, Shin-Woo (Department of Agronomy & Medicinal Plant Resources, Gyeongnam National University of Science & Technology)
Publication Information
Journal of Plant Biotechnology / v.45, no.4, 2018 , pp. 364-368 More about this Journal
Abstract
The R2R3-type protein IbMYB1 transcription factor is a key regulator for anthocyanin biosynthesis in the storage roots of sweet potatoes. It was previously demonstrated that the IbMYB1 expression stimulates anthocyanin pigmentation in tobacco leaves, arabidopsis and storage roots of sweet potatoes. In this study, we generated the transgenic potato plants that express the IbMYB1 genes, which accumulated high levels of anthocyanins under the control of either the tuber-specific patatin (PAT) promoter or oxidative stress-inducible peroxidase anionic 2 (SWPA2) promoter. The PAT-MYB1 transgenic lines exhibited higher anthocyanin levels in the tuber than the empty vector control (EV) or SWPA2-MYB1 plants. When combined, our results indicated that overexpression of the IbMYB1 is a highly promising strategy for the generation of transgenic plants with enhanced tissue specific anthocyanin production.
Keywords
Anthocyanin; IbMYB1; Transgenic potato; Tuber specific promoter;
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