Browse > Article
http://dx.doi.org/10.5010/JPB.2018.45.2.125

Microtuberization and Acclimatization in the Dioscorea cayenensis Thunb. by the Carbon Source  

Lee, Na Nyum (Forest Biotechnology Division, National Institute of Forest Science (NIFoS))
Kim, Ji Ah (Forest Biotechnology Division, National Institute of Forest Science (NIFoS))
Kim, Yong Wook (Special Forest Products Division, National Institute of Forest Science (NIFoS))
Kim, Tae Dong (Forest Biotechnology Division, National Institute of Forest Science (NIFoS))
Publication Information
Journal of Plant Biotechnology / v.45, no.2, 2018 , pp. 125-130 More about this Journal
Abstract
In this experiment, we investigated the effects of various carbon sources and concentrations on the microtuber induction and acclimatization of the yam (Dioscorea cayenensis). First, the effects of the in vitro carbon sources and concentrations on the microtuber induction were examined. The highest efficiency of the microtuber induction was obtained in the 7% sucrose treatment, whereas the glucose treatment shows no effect on the microtuber formation. Secondly, the effects of the survival rate and the microtuber formation rate after the acclimatization were examined. The diameter (6.1 mm) and fresh weight (0.5g) of the tuberous root are the highest in the pretreatment of the 7% sucrose. Although the survival rate of the pretreatment of the low concentration sucrose (3% sucrose) is 100 %, the growth and development were inhibited. These results suggest the 7% sucrose treatment is appropriate for the yam microtuber formation and acclimatization. In addition, this protocol could be used for the propagation of virus- or disease-free clones and the multiplication of elite yam cultivars.
Keywords
Dioscorea cayenensis; microtuberization; carbohydrate;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Alizadeh S, Mantell SH, MariaViana A (1998) In vitro shoot culture and microtuber induction in the steroid yam Dioscorea composite Hemsl. Plant Cell Tissue Organ Cult 53:107-112   DOI
2 Ascough GD, Erwin JE, van Staden J (2008) Reduced temperature, elevated sucrose, continuous light and gibberellic acid promote corm formation in Watsonia vanderspuyiae. Plant Cell Tissue Organ Cult 95:275-283   DOI
3 Balogun M, Fawole I, Ng S, Ng N, Shiwachi H, Kikuno H (2006) Interaction among cultural factors in microtuberization of white yam (Dioscorea rotundata). Trop Sci 46:55-59   DOI
4 Chen FQ, Fu Y, Wang DL, Gao X, Wang L (2007) The effect of plant growth regulators and sucrose on the micropropagation and microtuberization of Dioscorea nipponica Makino. J Plant Growth Regul 26:38-45   DOI
5 Conner AJ, Falloon PG (1993) Osmotic versus nutritional effects when rooting in vitro asparagus minicrown on high sucrose media. Plant Sci 89:101-106   DOI
6 Feng F, Ye CH, Li YZ, Xu WF (2007) Effects of growth regulators, carbon sources and photoperiod on in vitro formation and growth and development of microtubers of Dioscorea fordii Prain et Burk. Plant Physiol Commun 43:1045-1049
7 Huang CH, Chung JP (2011) Efficient indirect induction of protocorm-like bodies and shoot proliferation using field-grown axillary buds of a Lycaste hybrid. Plant Cell Tissue Organ Cult 106:31-38   DOI
8 International Institute for Tropical Agriculture (IITA) and Natural Resources Institute (NRI) (2006) "On the bright side of yam production" Retrieved on January 29, 2009 from: www.new-ag.info/06-3/develop/dev03.html
9 John JL, Courtney WH, Decoteau DR (1993) The influence of plant growth regulators and light on microtuber induction and formation in Dioscorea alata L. cultures. Plant Cell Tissue Organ Cult 34:245-252   DOI
10 Kim Ji Ah, Heung Kyu Moon, Yong Eui Choi (2013) Microtuber formation from in vitro Codonopsis lanceolata plantlets by sugar. J Plant Biotechnol 40:147-155   DOI
11 Li M., J Li, W Liu, L Liu, J Lu, J Niu, X Liu and Q Yang (2014). A protocol for In vitro production of microtubers in Chinese yam (Dioscorea opposita). Biosci Biotechnol Biochem 78:1005-1009   DOI
12 Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473-479   DOI
13 Ng CY, Saleh NM (2011) In vitro propagation of Paphiopedilum orchid through formation of protocorm - like bodies. Plant Cell Tissue Organ Cult 105:193-202   DOI
14 Olivier KA, Konan KN, Anike FN, Agbo GN, Dodo HW (2012) In vitro induction of minitubers in yam (Dioscorea cayenensis-D. rotundata complex). Plant Cell Tissue Organ Cult 109:179-189   DOI
15 Ovono PO, Kevers C, Dommes J (2007) Axillary proliferation and tuberisation of Dioscorea cayenensis-D. rotundata complex. Plant Cell Tissue Organ Cult 91:107-114   DOI
16 Rayirath UP, Lada RR, Caldwell CD, Samuel K, Asiedu SK, Kevin J, Sibley KJ (2011) Role of ethylene and jasmonic acid on rhizome induction and growth in rhubarb (Rheum rhabarbarum L.). Plant Cell Tissue Organ Cult 105:253-263   DOI
17 Torres-Moran MI, Martha Escoto-Delgadillo, Sandy Molina-Moret, Diana M. Rivera-Rodriguez, Ana P. Velasco-Ramirez, Diogenes Infante, Liberato Portillo (2010) Assessment of genetic fidelity among Agave tequilana plants propagated asexually via rhizomes versus in vitro culture. Plant Cell Tissue Organ Cult 103:403-409   DOI
18 Sheela Chandra, Rajib Bandopadhyay, Vijay Kumar, Ramesh Chandra (2010) Acclimatization of tissue cultured plantlets: from laboratory to land. Biotechnol Lett 32:1199-1205   DOI
19 Shou Yong Choy Ng (1988) In vitro tuberization in white yam (Dioscorea rotundata Poir). Plant Cell Tissue Organ Cult 14:121-128   DOI
20 Thorpe T, Stasolla C, Yeung EC, de Klerk GJ, Roberts A, George EF (2007) Plant propagation by tissue culture 3rd edition, p115-174. In: E.F. George MA Hall, and GJ de Kler (eds). Chapter 4. The components of plant tissue culture media II: organic additions, osmotic and pH effects, and support systems. Springer Dordrecht, Netherlands
21 Uchendu EE, Shukla M, Saxena PK, Joachim E. R. Keller (2016) Cryopreservation of potato microtubers: the critical roles of sucrose and desiccation. Plant Cell Tissue Organ Cult 124:649-656   DOI
22 Yan H, Yang L, Li Y (2011) Axillary shoot proliferation and tuberization of Dioscorea fordii Prain et Burk. Plant Cell Tissue Organ Cult 104:193-198   DOI
23 Yuki M, Michihiko S, Kenji K , Nobukazu N , Mari S, Misaki I, Yuichi K, Yoshiyuki M, Hiroyuki K, Kanako K, Tsutomu K, Akihito W, Hajime O, Hiroshi I, Nobuhiro M, Kunihiro N, Yu S & Takuji S (2015) The radish genome and comprehensive gene expression profile of tuberous root formation and development. Scientific Reports 5:10835, DOI: 10.1038/srep10835   DOI