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http://dx.doi.org/10.5010/JPB.2017.44.4.454

Conditions of In Vitro Spore Germination and Prothallium Culture for Sporophyte propagation of Polystichum braunii (Spenn.) Fée  

Kwon, Hyuk Joon (National Institute of Biological Resources)
Han, Ji Hyun (Brain Korea 21 Center for Bio-Resource Development, Division of Animal, Horticultural, and Food Sciences, Chungbuk National University)
Lee, Cheol Hee (Brain Korea 21 Center for Bio-Resource Development, Division of Animal, Horticultural, and Food Sciences, Chungbuk National University)
Kim, Soo-Young (National Institute of Biological Resources)
Publication Information
Journal of Plant Biotechnology / v.44, no.4, 2017 , pp. 454-461 More about this Journal
Abstract
This study was conducted to investigate the optimal conditions for spore germination, prothallus propagation, sporophyte formation, and seedling growth in Polystichum braunii (Spenn.) $F{\acute{e}}e$. The rate of spore germination and early prothalium development was high in Knop (41.2%), which had low mineral content. The optimal medium for prothallus propagation and sexual organ formation was 2MS medium (2% sucrose). Among the various mixtures of cultivation soil (bedding soil, peat moss, perlite, and decomposed granite), a mixture of bedding soil and decomposed granite at a ratio of 2:1 (v:v) had a positive effect on sporophyte formation (276.3 ea/$7.5m^2$). The most efficient conditions for promoting the growth of sporophytes were pots filled with only bedding soil.
Keywords
Braun's holly fern; Fern; Masspropagation; Medium type; Prickly shield fern; Pteridophyta;
Citations & Related Records
Times Cited By KSCI : 5  (Citation Analysis)
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