Browse > Article
http://dx.doi.org/10.5010/JPB.2017.44.3.330

Plant Regeneration via Adventitious Shoot Formation in Platycodon grandiflorum (Jacq. A. DC.)  

Kim, Ju Young (Department of Oriental Pharmaceutical Development, Medicinal Plant Transformation Center, Nambu University)
Na, Hyun Sun (Department of Oriental Pharmaceutical Development, Medicinal Plant Transformation Center, Nambu University)
Choi, Pil Son (Department of Oriental Pharmaceutical Development, Medicinal Plant Transformation Center, Nambu University)
Publication Information
Journal of Plant Biotechnology / v.44, no.3, 2017 , pp. 330-334 More about this Journal
Abstract
To investigate optimal conditions for plant regeneration in Platycodon grandiflorum (Jacq. A. DC.).Both leaf and hypocotyl explants were cultured on Murashige& Skoog's (MS) medium supplemented with combinations of 0.1, 0.5, 1.0, or 2.0 mg/L cytokinins (BA and kinetin) and 1.0 mg/L 2,4-D for 6 weeks, respectively. According to the type of explant, the total shoot organogenesis (56.38%) in leaf explants was higher than in hypocotyls (28.20%). In comparison with kinetin and BA for the plant regeneration, the frequency (70.38%) of leaf explants was higher in combination with kinetin and 2,4-D than of BA with 2,4-D (42.38%), whereas the frequency (35.56%) of hypocotyls explants was higher in BA combination than kinetin combination (20.83%). Thehighest frequency (94.20%) was observed from the cultures of leaf explants on the MS medium supplemented with 1.0 mg/L kinetin and 1.0 mg/L 2,4-D. Upon transfer onto 1/2 MS basal medium containing 3% sucrose, shoots developed into plantlets with roots, and were well grown in soil in the greenhouse. These results lead us to speculate that the optimization of culture conditions was responsible for the mass propagation from in vitro cultures of Platycodon grandiflorum (Jacq. A. DC.).
Keywords
Benzyl adenine; 2,4-dichlorophenoxyacetic acid; Hypocotyl; Kinetin; Leaf; Organogenesis; Platycodon grandiflorum;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
연도 인용수 순위
1 Choi PS, Komatsuda T, Kim MH, Choi KM, Choi DW, Liu JR (2002) Screening of soybean recombinant inbred lines for high competence somatic embryogenesis. Kor J Plant Biotechnol 29:135-138   DOI
2 Choi SR, Kim MJ, Eun JS, Ahn MS, Lim HS, Ryu J, You DH (2004) Effects of membrane filter and sucrose concentrations on the growth of balloon flower (Platycodon geandiflorum A. DC.) plantlets in vitro. Kor J Plant Biotechnol 31:209-217   DOI
3 Chung JH, Cho SH (2002) Tissue cultures of Platycodon grandiflour. J Agricult Life Sci 36:9-18
4 Eun JS, Kim YS, Kim YH (2000) Effects of light emitting diodes on growth and morphogenesis of in vitro seedling in Platycodon geandiflorum. Kor J Plant Tiss Cult 27:71-75
5 Erisen S, Yorgancilar M, Atalay E, Babaoglu M, Duran A (2010.) Callus induction and plant regeneration of endemic Astragalus nezaketae in Turkey. Electron of Biotechnol. DOI: 10.2225/vol13-issue6-fulltex-3
6 Gill R, Saxena PK (1992) Direct somatic embryogenesis and regeneration of plants from seedling explants of peanut (Arachis hypogaea): promotive role of thidiazuron. Can J Bot 70:1186-1192   DOI
7 Han C, Lee Y (1976) Induction of plantlets in the anther culture of Platycodon grandiflorum. Kor J. Plant Tiss Cult 4:1-3
8 Han EH, Son YW, Kim MB, Shin YW, Cho YS, Lee SW (2014) Establishment of tissue culture and acclimation of white ballon flower (Platycodon grandiflorum DC. cv. Jangback) for the eaising of in vitro propagated seedlings. J Plant Biotechnol. 41:134-139   DOI
9 Hou SW, Jia JF (2004) High frequency plant regeneration from Astragalus melilotoides hypocotyl and stem explants via somatic embryogenesis and organogenesis. Plant Cell Tiss Org Cult 79(1):95-100   DOI
10 Kasumi M, Suzuki K, Gonai T, Nog M, Yamafa T, Takatsu Y (2006) Adventitious buf formation and plant regeneration from ovary explants in balloon flower (Platycodon geandiflorum). J Ja Soc Hort Sci 75:284-286   DOI
11 Kim SW, Liu JR (1999) Somatic embryogenesis and plant regeneration in zygotic embryo cultures of balloon flower. Plant Cell Tiss Org Cult 58:227-230.   DOI
12 Ko JA (1999) Effect of low temperature pretreatment on pollen dimorphism and embryo formation in anther culture of Platycodon grandiflorum. Kor J Plant Tiss Cult 26:149-156
13 Ko JA, Kim YS, Kim MJ, Eun JS (1993) Somatic embryogenesis and plant regeneration from leaf and stem culture of Platycodon grandiflorum. Kor J. Breeding 25:337-343
14 Lee BK, Ko JA, Kim YS, Eun JS (1993) Embryogenesis and plnat regeneration in anther cultures of Platycodon grandiflorum. Kor J Plant Tiss Cult 20:153-157
15 Lee SW, Son YW, Shin YW, Cho YS (2014) The current status in Korea and future perspectives of ballon flower (Platycodon grandiflorum) in Gyeongsangnam-do. Kor J Int Agric 26:68-72   DOI
16 Malik KA, Saxena PK (1992) Regeneration in Phaseoulus vulgaris seedling by $N^6$-benzylaminopurine and thidiazuron. Planta 186:384-389
17 Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue culture. Physiol Plant 15:473-479   DOI
18 Park MS, Choi PS (2015) Plant regeneration from hypocotyl explants of Astragalus sinicus L. J Plant Biotechnol 42:396-400   DOI
19 Soh WY, Cho DY, Lee EK (1996) Multicotyledonary structure of somatic embryo formed cell cultures of Daucus carota L. Korean J Bot 39:71-79
20 Turgut-Kara N, ARI S (2008) In vitro plant regeneration from embryogenic cell suspension culture of Astragalus chrysochlorus (Leguminoseae). Afr J Biotechnol 7:1250-1255
21 Cho HJ, Widholm JM (2002). Improved shoot regeneration protocol for hairy roots of the legume Astragalus sinicus. Plant Cell, Tiss Org Cult 69(3):259-269   DOI
22 Wright MS, Koehler SM, Hinchee MA, Carnes MG (1986) Plant regeneration by organogenesis in Glycine max. Plant Cell Rep 5:150-154   DOI
23 Barwale UB, Meyer MM, Widholm JM (1986) Screening of Glycine max and Glycine soja genotypes for multiple shoot formation at the cotyledonary node. Theor. Appl. Genet. 72:423-428   DOI
24 Cho DY, Lee EK, Soh WY (1998) Plant regeneration from somatic embryo with structural diversity from leaf explants cultures of Osteicum koreanum Kitagawa. Kor J Plant Tiss Cult 25:51-56