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http://dx.doi.org/10.5010/JPB.2016.43.2.223

Proteomic analysis of dehydroascorbate reductase transgenic potato plants  

Han, Eun-Heui (Department of Agronomy & Medicinal Plant Resources, Gyeongnam National University of Science & Technology)
Goo, Young-Min (Gyeongnam Oriental Medicinal Herb Institute)
Kim, Yun-Hee (Department of Biology Education, College of Education, IALS, Gyeongsang National University)
Lee, Shin-Woo (Department of Agronomy & Medicinal Plant Resources, Gyeongnam National University of Science & Technology)
Publication Information
Journal of Plant Biotechnology / v.43, no.2, 2016 , pp. 223-230 More about this Journal
Abstract
Ascorbic acid (AsA) is a strong antioxidant/reducing agent that can be converted to dehydroascorbate (DHA) by oxidation in plants. DHA, a very short-lived chemical, is recycled to AsA by dehydroascorbate reductase (DHAR). Previously, DHAR cDNA was isolated from the hairy roots of the sesame plant, and DHAR-overexpressing transgenic potato plants were generated under the control of the CaMV35S promoter (CaMV35S::DHAR). An increase in transgene expression and ascorbate levels were observed in the transgenic plants. In the present study, proteomic analysis revealed that transgenic plants not only accumulated DHAR in their cells, but also induced several other antioxidant enzyme-related proteins during plant growth. These results suggest that DHAR is important for stress tolerance via induction of antioxidant proteins, and could improve stress tolerance in transgenic potato plants.
Keywords
Potato; Dehydroascorbate reductase; Proteomics; Antioxidant enzyme; Ascorbate;
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