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http://dx.doi.org/10.5010/JPB.2015.42.3.245

Effect of LED mixed light conditions on the glucosinolate pathway in brassica rapa  

Moon, Junghyun (Functional Biomaterial Division, National Academy of Agricultural Science, Rural Development Administration)
Jeong, Mi Jeong (Functional Biomaterial Division, National Academy of Agricultural Science, Rural Development Administration)
Lee, Soo In (Functional Biomaterial Division, National Academy of Agricultural Science, Rural Development Administration)
Lee, Jun Gu (Department of Horticulture, Chonbuk National University)
Hwang, Hyunseung (Department of Plant Science, College of Agriculture and Life Sciences, Seoul National University)
Yu, Jaewoong (Department of Molecular Biotechnology, Konkuk University)
Kim, Yong-Rok (Department of Chemistry, Yonsei University)
Park, Se Won (Department of Molecular Biotechnology, Konkuk University)
Kim, Jin A (Functional Biomaterial Division, National Academy of Agricultural Science, Rural Development Administration)
Publication Information
Journal of Plant Biotechnology / v.42, no.3, 2015 , pp. 245-256 More about this Journal
Abstract
In the agricultural industries, LEDs are used as supplementary, as well as main lighting sources in closed cultivation systems. In cultivation using artificial light sources, various light qualities have been tried to supplement fluorescent lamps to promote plant growth and metabolism. Microarray analysis of Brassica rapa seedlings under blue and fluorescent mixed with blue light conditions identified changes in three genes of the glucosinolate pathway. This attracted attention as functional materials highly expressed 3.6-4.6 fold under latter condition. We selected four more genes of the glucosinolate pathway from the Brassica database and tested their expression changes under fluorescent light mixed with red, green, and blue, respectively. Some genes increased expression under red and blue mixed conditions. The Bra026058, Bra015379, and Bra021429; the orthologous genes of CYP79F1, ST5a, and FMOGS-OX1 in Arabidopsis, are highly expressed in Brassica rapa under fluorescent mixed with blue light conditions. Further, Bra029355, Bra034180, Bra024634, and Bra022448; the orthologous genes of MAM1, AOP3, UGT74B1, and BCAT4 in Arabidopsis, are highly expressed in Brassica rapa under fluorescent mixed with red light conditions. The various light conditions had unique effects on the varieties of Brassica, resulting in differences in glucosinolate synthesis. However, in some varieties, glucosinolate synthesis increased under mixed blue light conditions. These results will help to construct artificial light facilities, which increase functional crops production.
Keywords
Blue light; Brassica rapa; Glucosinolate; LED; Microarray;
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