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http://dx.doi.org/10.5010/JPB.2015.42.3.204

Effects of Brassica rapa SHI-RELATED SEQUENCE overexpression on petunia growth and development  

Hong, Joon Ki (National Academy of Agricultural Science, Rural Development Administration)
Suh, Eun Jung (National Academy of Agricultural Science, Rural Development Administration)
Lee, Su Young (Floricultural Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration)
Song, Cheon Young (Department of Floriculture, Korea National College of Agriculture and Fisheries)
Lee, Seung Bum (National Academy of Agricultural Science, Rural Development Administration)
Kim, Jin A (National Academy of Agricultural Science, Rural Development Administration)
Lee, Soo In (National Academy of Agricultural Science, Rural Development Administration)
Lee, Yeon-Hee (National Academy of Agricultural Science, Rural Development Administration)
Publication Information
Journal of Plant Biotechnology / v.42, no.3, 2015 , pp. 204-214 More about this Journal
Abstract
SHI-RELATED SEQUENCE (SRS) genes are plant-specific transcription factors that contain a zinc-binding RING finger motif, which play a critical role in plant growth and development. Among Brassica rapa SRS genes, BrSRS7 and BrLRP1 genes, isolated from shoot apical regions are important regulators of plant growth and development. In order to explore the function of BrSRS genes in horticultural plant growth and development, two constructs containing BrSRS7 and BrLRP1 under the control of a cauliflower mosaic virus 35S promoter were introduced into petunia by Agrobacterium-mediated transformation. The resulting transgenic plants were dwarf and compact plants with reduced plant height and diameter. Additionally, these transgenic plants had upward-curled leaves of narrow width and short internodes. Interestingly, the flower shapes of petunia were different among transgenic plants harboring different kinds of SRS genes. These phenotypes were stably inherited through generations $T_2$ and $T_3$. Semi-quantitative RT-PCR analyses of transgenic plants revealed that BrSRS7 and BrLRP1 regulate expression of gibberellin (GA)- and auxinrelated genes, PtAGL15- and PtIAMT1-related, involved in shoot morphogenesis. These results indicate that the overexpression of BrSRS7 and BrLRP1 genes suppressed the growth and development of petunia by regulating expression of GA- and auxin-related genes. From these data, we deduce that BrSRS7 and BrLRP1 genes play an important role in the regulation of plant growth and development in petunia. These findings suggest that transformation with the BrSRS genes can be applied to other species as a tool for growth retardation and modification of plant forms.
Keywords
Short internodes; Transcription factor; Transgenic plant; Retardation; Morphogenesis;
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Times Cited By KSCI : 2  (Citation Analysis)
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