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http://dx.doi.org/10.5010/JPB.2015.42.1.6

Development of molecular biological techniques for the differentiation of medicinal plant species  

Han, Eun-Heui (Dept. of Agronomy & Medicinal Plant Resources, Gyeongnam National University of Science & Technology)
Kim, Yun-Hee (Dept. of Biology Education, College of Education, IALS, Gyeongsang National University)
Lee, Shin-Woo (Dept. of Agronomy & Medicinal Plant Resources, Gyeongnam National University of Science & Technology)
Publication Information
Journal of Plant Biotechnology / v.42, no.1, 2015 , pp. 6-12 More about this Journal
Abstract
Medicinal plants resources are becoming important assets since their usages have been expanded to the development of functional foods for human health, more attractive cosmetics, and pharmaceutical industries. However, their phylogenetic origins and names are different from each country and quite often they are mixed each other resulting in the confusion for consumers. In particular, when they are very similar based on their morphological characteristics and distributed as dried roots, it is extremely difficult to differentiate their origins even by specialists. Recently, "DNA barcodes" have been extensively applied to identify their origin of medicinal plant species. In this review, we tried to overview the current research achievements for the development of suitable "DNA barcodes" regarding to the differentiation of medicinal plant species. Furthermore, more advanced techniques including amplification refractory mutation system (ARMS)-PCR, multiplex single base extension (MSBE), high-resolution melting (HRM) curve analyses are also discussed for their practical applications in the authentification of particular medicinal plant species.
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1 Arnot DE, Roper C, Bayoumi RA (1993) Digital codes from hypervariable tandemly repeated DNA sequences in the Plasmodium falciparum circumsporozoite gene can genetically barcode isolates. Mol Biochem Parasitol 61:15-24   DOI
2 Baigalmaa J, Kim MK, Noh JH, Hua S, Yang DC (2009) Phylogenetic analysis of Schizonepeta Spike on the basis of DNA sequences. K J Med Crop Sci 17:46-53
3 CBOL Plant Working Group (2009) A DNA barcode for land plants. Proc Natl Acad Sci 106:12794-12797   DOI
4 Chase MW, Cowan RS, Hollingsworth PM, van den Berg C, Madrinan S, Petersen G, Seberg O, Jorgsensen T, Cameron KM, Carine M, Pedersen N, Hedderson TAJ, Conrad F, Salazar GA, Richardson JE, Hollingsworth ML, Barraclough TG, Kelly L, Wilkinson M (2007) A proposal for a standardised protocol to barcode all land plants. Taxon 56: 295-299
5 Chen S, Yao H, Han J, Liu C, Song J, Shi L, Zhu Y, Ma X, Gao T, Pang X, Luo K, Li Y, Li X, Jia X, Lin Y, Leon C (2010) Validation of the ITS2 region as a novel DNA barcode for identifying medicinal plant species. PLoS One 5:1-8, e8613
6 Dunning LT, Savolainen V (2010) Broad-scale amplification of matK for DNA barcoding plants, a technical note. Botanical J of the Linnean Society 164:1-9   DOI
7 Fushimi H, Komatsu K, Isobe M, Namba T (1997) A new approach for the identification of a Chinese traditional medicine, "Chuanxiong" by 18S ribosomal RNA gene sequences. Phytomedicin 3:387-389   DOI
8 Gao T, Yao H, Song J, Liu C, Zhu Y, Ma X, Pang X, Xu H, Chen S (2010) Identification of medicinal plants in the family Fabaceae using a potential DNA barcode ITS 2. J Ethnopharmacology 130:116-121   DOI
9 He Y, Hou P, Fan G, Song Z, Liu H, Li Y, Zhang Y (2011) Internal transcribed spacers (ITS) identification of Angelica anomala Lallem Chuanbaizhi (in Chinese) cultivars collected in Sichuan and their molecular phylogenetic analysis with other Angelica L. species. J Med Plants Res 5:3653-3659
10 Hebert PDN, Cywinska A, Ball SL, de Waard JR (2003) Biological identifications through DNA barcodes. Proc Royal Soc London, series B 270:313-321   DOI
11 Hebert PDN, Ratnasingham S, de Waard JR. (2003) Barcoding animal life: cytochrome c oxidase subunit 1 divergences among closely related species. Proc Biol Sci 270:S96-99   DOI
12 Hollingsworth PM, Graham SW, Little DP (2011) Choosing and using a plant DNA barcode. PLoS One 6:e19254   DOI
13 Jaakola L, Suokas M, Haggman (2010) Novel approaches based on DNA barcoding and high-resolution melting of amplicons for authenticity analyses of berry species. Food Chem 123:492-500
14 Kalivas A, Ganopoulos I, Xanthopoulou A, Chatzopoulou P, Tsaftaris A, Madesis P (2014) DNA barcode ITS2 coupled with high resolution melting (HRM) analysis for taxonomic identification of Sideritis species growing in Greece. Mol Biol Rep 41:5147-155   DOI
15 Kim JH, Jung JY, Choi HI, Kim NH, Park JY, Lee Y, Yang TJ (2013) Diversity and evolution of major Panax species revealed by scanning the entire chloroplast intergenic spacer sequences. Genet Resour Crop Evol 60:413-25   DOI
16 Kim YH, Choi G, Lee HW, Lee GH, Chae SW, Kim YH, Lee MY (2012) Comparison of Angelica species roots using taste sensor and DNA sequencing analysis. Kor J Herbology 27:37-42
17 Kress WJ, Erickson DL (2007) A two-locus global DNA barcode for land plants: The coding rbcL gene complements the non-coding trnH-psbA spacer region. PLoS ONE 2: e508.   DOI
18 Little S (1994) Amplification-refractory mutation system (ARMS) analysis of point mutations. In. current protocols in human molecular genetics. (Eds. Nicholas C Dracopoli et al.) Jhon Wiley & Sons, IOOnc. New York, pp9.8.1.- 9.8.12
19 Kuo LY, Li FW, Chiou WL, Wang CN (2011) First insights into fern matK phylogeny. Mol Phylo Evol 59:556-566   DOI
20 Lee YM, Moon BC, Ji Y, Kim WJ, Kim HK (2013) Molecular Authentication of Pinelliae Tuber from its adulterants by the analysis of DNA barcodes, matK and rbcL genes. Kor. J. Herbology 2013;28(6):53-58   DOI
21 Liu YP, Cao H, Han GR, Fushimi H, Komatsu K (2002) MatK ITS nucleotide sequencing of crude drug chuanxiong and phylogenetic relationship between their species from China and Japan. Yao Xue Xue Bao 37:63-68
22 Mader E, Ruzicka J, Schmiderer C, Novak J (2011) Quantitative high-resolution melting analysis for detecting adulterations. Anal Biochem 409:153-155   DOI
23 Moon BC, Lee YM, Ji Y, Choi G, Chun JM, Kim HK (2013) Molecular authentication and phylogenetic analysis of plant species for Breeae and Cirsii Herba based on DNA barcodes. Kor J Herbology 28:75-84
24 Newton CR, Graham A, Heptinstall LE (1989) Analysis of any point mutation in DNA. The amplification refractory mutation system(ARMS). Nucl Acids Res 17:2503-2516   DOI
25 Ohsako T, Ohnishi O (2001) Nucleotide sequence variation of the chloroplast trnK/matK region in two wild Fagopyrum (Polygonaceae) species, F. leptopodum and F. statice. Genes Genet Syst 76:39-46   DOI
26 Park YJ, Kwon SO, Park JW (2014) Identification method of Liriope species and identification probe thereof. Korea patent 10-2014-0038154
27 Song IG, An BR, Seo BI, Park SJ (2009) Molecular marker to identify and origin of Cnidii Rhizoma from Korea and China. Kor J Herbology 24:1-8
28 Piredda R, Simeone MC, Attimonelli M, Bellarosa R, Schirone B (2010) Prospects of barcoding the Italian wild dendroflora: oaks reveal severe limitations to tracking species identity. Mol Eco Ress 11:72-73
29 Ren BQ, Xiang XG, Chen ZD (2010) Species identification of Alnus (Betulaceae) using nrDNA and cpDNA genetic markers. Mol Eco Res 10: 594-605
30 Roy S, Tyagi A, Shukla V, Kumar A, Singh UM, et al. (2010) Universal plant DNA barcode loci may not work in complex groups: A case study with Indian Berberis species. PLoS ONE 5: e13674   DOI
31 Sun XQ, Bai MM, Yao H, Gao JI, Li MM, Hang YY (2013) DNA barcoding of populations of Fallopia multiflora, an indigenous herb in China. Genet Mol Res 12:4078-4089   DOI
32 Valentini A, Pompanon F, Taberlet P (2008) DNA barcoding for ecologists. Trends Ecol Evol 24:110-130
33 Valentini A, Miquel C, Nawaz MA, Bellemain EVA, Coissac E, Pompanon F, Gielly L, Ruaud C, Nascetti G, Wincker P, Swenson JE, Taberlet P (2009) New perspectives in diet analysis based on DNA barcoding and parallel pyrosequencing: the trnL approach. Mol Ecol Res 9:51-60
34 Vietina M, Agrimonti C, Marmiroli N (2013) Detection of plant oil DNA using high resolution melting (HRM) post PCR analysis: A tool for disclosure of olive oil adulteration. Food Chem 141:3820-3826   DOI
35 von Crautlein M, Korpelainen H, Pietilainen M, Rikkinen J (2011) DNA barcoding: a tool for improved taxon identification and detection of species diversity. Biodiversity and Conservation 20:373-89.   DOI
36 Yao H, Song J, Liu C, Luo K, Han J, Li Y, Pang X, Xu H, Zhu Y, Xiao P, Chen S (2010) Use of ITS2 region as the universal DNA barcode for plants and animals. PLoS ONE 5:e13102   DOI
37 Wicke S, Quandt D (2009) Universal primers for amplification of the trnK/matK region in land plants. Anales del Jardin Botanico de Madrid 66:285-88.   DOI
38 Yan P, Pang QH, Jiao XW, Zhao X, Shen YJ, Zhao SJ (2008) Genetic variation and identification of cultivated Fallopia multiflora and its wild relatives by using chloroplast matK and 18S rRNA gene sequences. Planta Med 74:1504-1509   DOI
39 Yang DC, Wang HD (2012) SNP primers for Gumpoong and Chungsun selection of Korean ginseng (Panax ginseng C.A. Meyer) and method for Gumpoong and Chungsun distinciton using the same. KR patent 10-2012-0106414
40 Zheng CJ, Zhao SJ, Zhao ZH, Guo J (2009) Molecular authentification of the traditional medicinal plant Fallopia multiflora. Planta Med 75:870-872   DOI
41 Zhu S, Fushimi H, Han G, Tsuchida T, Uno T, Takano A, Komatsu K, (2007) Molecular identification of "Chuanxiong" by nucleotide sequence and multiplex single base extension analysis on chloroplast trnK gene. Biol Pharm Bull 30:527-531   DOI