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http://dx.doi.org/10.5010/JPB.2014.41.3.140

Efficient shoot regeneration using cotyledon explants and Agrobacterium tumefaciens - mediated transformation of bottle gourd (Lagenaria sicraria Standl.)  

Kim, Soo-Yun (Vegetable Research Division, National Institute of Horticultural & Herbal Science, Department of Life Sciences, RDA)
Ahn, Yul-Kyun (Vegetable Research Division, National Institute of Horticultural & Herbal Science, Department of Life Sciences, RDA)
Huh, Yun-Chan (Vegetable Research Division, National Institute of Horticultural & Herbal Science, Department of Life Sciences, RDA)
Lee, Hye-Eun (Vegetable Research Division, National Institute of Horticultural & Herbal Science, Department of Life Sciences, RDA)
Kim, Do-Sun (Vegetable Research Division, National Institute of Horticultural & Herbal Science, Department of Life Sciences, RDA)
Publication Information
Journal of Plant Biotechnology / v.41, no.3, 2014 , pp. 140-145 More about this Journal
Abstract
This study were carried out for selection of proper transformation variety and development of efficient regeneration and transformation methods. The number of shoot in commercial varieties of gourd plant were 0 ~ 7.3. and fusarium wilt resistant pure lines were 2.0 ~ 6.5 per dish containing on MS medium supplemented with 3 mg/L BA. The shoot regeneration frequency of fusarium wilt resistant pure lines were wide variation on the deviation. The expression of GFP was high 67% and 100% at the co-cultivation with Agrobacterium. The effective shoot regeneration plant hormone were combination BA and 2,4-D. The number and elongation condition of shoot was good after 4 weeks change with MS medium supplemented with 1 mg/L BA. Effective callus production plant hormone were combination of 3 mg/L BA and 0.1 mg/L 2.4-D.
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