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http://dx.doi.org/10.5010/JPB.2014.41.2.89

New embryogenesis from atypical bodies and plant regeneration from long-term subcultured embryogenic callus in rose  

Lee, Su Young (National Institute of Horticultural & Herbal Science, Rural Development Administration)
Do, Kyoung Ran (National Institute of Horticultural & Herbal Science, Rural Development Administration)
Cheon, Kyeong-Seong (National Institute of Horticultural & Herbal Science, Rural Development Administration)
Kim, Won Hee (National Institute of Horticultural & Herbal Science, Rural Development Administration)
Kwon, O Hyeon (National Institute of Horticultural & Herbal Science, Rural Development Administration)
Lee, Hye Jin (National Institute of Horticultural & Herbal Science, Rural Development Administration)
Publication Information
Journal of Plant Biotechnology / v.41, no.2, 2014 , pp. 89-93 More about this Journal
Abstract
Long-term subcultured rose embryogenic calluses, which had been maintained for more than 5 to 6 years since the first embryogenesis from calluses induced from in vitro roots of rose, were identified as potential material for the development of transgenic plants. The first embryogenic calluses from 'Sweet Yellow' and two breeding lines (KR056002 and KR056006) were obtained in 2007 and 2009, respectively. Subsequently, we found that plants regenerated from long-term embryogenic calluses (LEC). Whereas the LEC from 'Sweet Yellow' takes 3 to 4 months to regenerate plants, those of the two breeding lines take 4 to 5 months. This period of time is the same as that taken for plants to regenerate from the first embryogenic callus. New embryogenesis was observed from atypical bodies (ABs) that appeared during the process of long-term subculture. We found that it is possible to use the AB as a material for new embryogenesis.
Keywords
Atypical body; Embryogenic callus; Long-term subculture; New embryogenesis regeneration; Rose;
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Times Cited By KSCI : 3  (Citation Analysis)
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