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http://dx.doi.org/10.5010/JPB.2014.41.1.44

Sodium hypochlorite treatment and light-emitting diode (LED) irradiation effect on in vitro germination of Oreorchis patens (Lindl.) Lindl  

Bae, Kee Hwa (National Institute of Biological Resources)
Oh, Kyoung Hee (National Institute of Biological Resources)
Kim, Soo-Young (National Institute of Biological Resources)
Publication Information
Journal of Plant Biotechnology / v.41, no.1, 2014 , pp. 44-49 More about this Journal
Abstract
In this study, we investigated the effects of sodium hypochlorite (NaOCl) and red or blue light-emitting diode (LED) light on embryo swelling and germination of Oreorchis patens (Lindl.) Lindl. A method for determining the swelling and protocorm formation in O. patens seeds through in vitro examination of immature seeds was established. NaOCl treatment of immature seeds was found to significantly enhance the extent of embryo swelling and protocorm formation in immature zygote embryos compared to those in the untreated controls. Additionally, the effects of white fluorescent light, and red and blue LED lights on embryo swelling and protocorm formation in in vitro cultured seeds were examined and compared to the conditions with or without NaOCl treatment. The most suitable light for embryo swelling and protocorm formation was the red LED light.
Keywords
Germination; Medium; Orchid; Oreorchis patens;
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Times Cited By KSCI : 2  (Citation Analysis)
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