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http://dx.doi.org/10.5010/JPB.2013.40.4.203

Shoot regeneration via culture of leaf explants in pear (Pyrus pyrifolia cv. Niitaka)  

Kim, Se Hee (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA)
Shin, Il Sheob (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA)
Cho, Kang Hee (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA)
Kim, Dae Hyun (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA)
Kim, Hyun Ran (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA)
Kim, Ki Ok (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA)
Lee, Hyang Bun (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA)
Do, Kyung Ran (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA)
Chun, Jae An (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA)
Hwang, Hae Seong (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA)
Publication Information
Journal of Plant Biotechnology / v.40, no.4, 2013 , pp. 203-209 More about this Journal
Abstract
Genetic manipulation of pear (Pyrus pyrifolia Nakai) breeding is still difficult due to lack of reliable regeneration system. The aim of this research is to establish shoot regeneration system from leaf explants for pear (P. pyrifolia cv. Niitaka) using various concentrations of plant growth regulators and carbon source supplemented to medium. The highest regeneration rate of about 20% was found on a medium containing 4.4 g/L of Murashige and Skoog (MS) without vitamins, Linsmaier and Skoog (LS) vitamins were added separately. Leaf explants of pear were cultured on MS medium containing 7 g/L of Daishin agar supplemented with various concentrations of NAA (0.01, 0.05, 0.1, 0.5 mg/L) in combination with BA(3, 5, 10 mg/L) for shoot regeneration. In medium with 5 mg/L of BA and 0.01 mg/L of NAA, adventitious shoot regeneration rate was higher than others treated. The optimal results were observed using MS medium supplemented with 30 g/L sorbitol as carbon source on regeneration system. Sorbitol is considered better carbon source than sucrose for shoot regeneration of pear (P. pyrifolia cv. Niitaka). In order to increase of shoot regeneration in pear (P. pyrifolia cv. Niitaka), plant agar and Daishin agar used as gelling agents, Daishin agar is more efficient in shoot regeneration.
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