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http://dx.doi.org/10.5010/JPB.2013.40.3.135

Transgene structures of marker-free transgenic Bt rice plants  

Woo, Hee-Jong (National Academy of Agricultural Science, Rural Development Administration (RDA))
Lee, Seung Bum (National Academy of Agricultural Science, Rural Development Administration (RDA))
Lim, Myung-Ho (National Academy of Agricultural Science, Rural Development Administration (RDA))
Gwon, Sun-Jong (National Academy of Agricultural Science, Rural Development Administration (RDA))
Lee, Jin-Hyoung (National Academy of Agricultural Science, Rural Development Administration (RDA))
Shin, Kong-Sik (National Academy of Agricultural Science, Rural Development Administration (RDA))
Cho, Hyun-Suk (National Academy of Agricultural Science, Rural Development Administration (RDA))
Publication Information
Journal of Plant Biotechnology / v.40, no.3, 2013 , pp. 135-140 More about this Journal
Abstract
A less simple approach developed for generation of marker-free transgenic plants is to select transformants without the use of selective marker genes. Some results about development of marker-free transgenic plants were obtained using a non-selective approach in several crops such as rice, potato and tobacco. However, the study did not provide evidence on detailed characterization of introduced gene on genome, a critical step for confirming the stable integration and transmission of a foreign gene. In this study, we evaluated structure and integration sites of transgene (mCry1Ac) in the transgenic Bt rice plants which were made via conventional Agrobacterium-mediated transformation by non-selective method. Structure and integration sites of transgene in these transgenic plants had similar fashion as those recovered under selection.
Keywords
Non-selective; Marker-free transgenic rice; Agrobacterium-mediated transformation; Transgene; integration site;
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