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http://dx.doi.org/10.5010/JPB.2012.39.1.081

Crop proteomics: Practical method for high resolution of two-dimensional electrophoresis  

Kim, U.G. (Department of Plant Bioscience, Pusan National University)
Jung, Hwa-Jin (Department of Plant Bioscience, Pusan National University)
Lee, Su-Ji (Department of Plant Bioscience, Pusan National University)
Kim, Sun-Tae (Department of Plant Bioscience, Pusan National University)
Publication Information
Journal of Plant Biotechnology / v.39, no.1, 2012 , pp. 81-92 More about this Journal
Abstract
Two-dimensional gel electrophoresis (2-DGE) is one of the most important technologies for high-resolution separation of proteins for proteomics. In this study, we present a detail 2-DGE protocol which allows detection and quantification of total plant proteins separated on gels to improve matching in image analysis. This protocol highlighted here may be useful for researchers, who like to first study for the development of protein biomarkers involved in development, biotic and abiotic stresses in plant.
Keywords
2-DGE; proteomics; biotic stresses; abiotic stresses; protein biomarker;
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