Browse > Article
http://dx.doi.org/10.5010/JPB.2011.38.3.215

Increase of resistance to oxidative stress induced by methyl viologen in progeny from a cross between two transgenic Petunia lines with NDPK and SOD genes  

Lee, Su-Young (National Institute of Horticultural & Herbal Science, Rural Development Administration)
Lee, Jung-Lim (National Institute of Horticultural & Herbal Science, Rural Development Administration)
Kim, Dool-Yi (Bio-Crop Development Division, National Academy Agricultural Science, Rural Development Administration)
Publication Information
Journal of Plant Biotechnology / v.38, no.3, 2011 , pp. 215-220 More about this Journal
Abstract
This study was conducted to investigate how to enhance resistance to oxidative stress in petunia progeny obtained by a crossing between transgenic plants, MnSOD (SOD2) ($T_4$) and NDPK2 ($T_2$), to develop transgenic petunia much more resistant to environmental stress. At the treatment of MV 200 ${\mu}M$, the progeny was significantly less damaged than its parental plants (SOD2- or NDPK2-transgenic lines) as well as wild type plants, implying its resistance to oxidative stress was enhanced compare to that of SOD2- or NDPK2- transgenic plants. In an expression of 11 quantitative traits, the progeny remained similar to control plants, although it infrequently displayed slightly longer or wider than either parental or wild type plants. In the expression of 6 qualitative traits, there was no significant difference between parental or non-transgenic control plants.
Keywords
Environmental stress; Morphological traits; PCR; Petunia hybrida;
Citations & Related Records
Times Cited By KSCI : 7  (Citation Analysis)
연도 인용수 순위
1 Tang L, Kwon SY, Kim MD, Kim JS, Kwak SS, Lee HS (2007) Enhanced tolerance to oxidative stress of transgenic potato (cv. Superior) plants expressing SOD and APX in chloroplasts. Kor J Plant Biotech 34:299-305   과학기술학회마을   DOI
2 Tang L, Kwon SY, Yun DJ, Kwak SS, Lee HS (2004a) Selection of transgenic potato plants expressing NDP Kinase 2 gene with enhanced tolerance to oxidative stress. Kor J Plant Biotech 31:19-195   과학기술학회마을
3 Tang L, Kwon SY, Kwak SS, Sung CK, Lee HS (2004b) Selection of transgenic potato plants expressing both CUZNSOD and APX in chloroplasts with enhanced tolerance to oxidative stress. Kor J Plant Biotech 31:109-113   과학기술학회마을   DOI
4 Tang, L, Kim MD, Yang KS, Kwon SY, Kim SH, Kim JS, Yun DJ, Kwak SS, Lee HS (2008) Enhanced tolerance of transgenic potato plants overexpressing nucleoside diphosphate kinase 2 against multiple environmental stresses. Transgenic Res 17: 705-715   DOI
5 Tseng MJ, Liu CW, Yiu JC (2008) Tolerance to sulfur dioxide in Chinese cabbage transformed with both the superoxide dismutase containing manganese and catalase genes of Escherichia coli. Sci Hort 115:101-110   DOI
6 Zhang Y,Yin X, Yang A, Li G, Zhang J (2005) stability of inheritance of transgenes in maize (Zea mays L.) lines produced using different transformation methods. Euphytica 144:11-22   DOI
7 Lee SY, Han BH, Cho AY (2009a) Inheritance and expression of transgene in SOD2-transgenic petunia descendants and their morphological traits. Kor J Plant Biotech 36:289-293   과학기술학회마을   DOI
8 Lee SY, Han BH,, Noh EW, Kwak SS (2009b) Transfer of SOD2 or NDP kinase 2 genes into purebred lines of petunia. Kor J Plant Biotech 36:144-148   과학기술학회마을   DOI
9 Lee SY, Han BH, Kim YT, Kim JS (2010) Resistance of SOD2-Transgenic Petunia Line to Oxidative Stress. Kor J Plant Biotech 37:532-566   과학기술학회마을   DOI
10 Lim S, Kim YH, Kim SH, Kwon SY, Lee HS, Kim JS, Cho KY, Paek KY, Kwak SS (2007) Enhanced tolerance of transgenic sweetpotato plants that express both CuZnSOD and APX in chloroplasts to methyl viologen-mediated oxidative stress and chilling. Mol Breeeding 19:227-239   DOI
11 Moon H, Lee B, Choi G, Shin D, Prasad DT, Lee O, Kwak SS, Kim DH, Nam J, Bahk J, Hong JC, Lee SY, Cho MJ, Lim CO, Yun DJ (2003) NDP kinase 2 interacts with two oxidative stressactivated MARKs to regulate cellular redox state and enhances multiple stress tolerance in transgenic plants. PNAS 100:358-363   DOI
12 National Seed Management Office (1997) Standard for survey in application and evaluation of carnation new varieties. pp 32-40 (In Korean)
13 Rural Development Administration (1995) Standard for survey in agricultural experiment & research. pp 388-390 (In Korean)
14 Sriskandarajah S, Mibus H, Serek M (2007) Transgenic Campanula carpatica plants with reduced ethylene sensitivity. Plant Cell Rep 26:805-813   DOI
15 Suntres Z (2002) Role of antioxidants in paraquat toxicity. Toxicology 180:65-77   DOI
16 Chareonpornwattana S, Thara KV, Wang L, Data SK, Panbangred W, Muthukrishnan S (1999) Inheritance, expression, and silencing of a chitinase transgene in rice. Theor Appl Genet 98:371-378   DOI
17 Choi HW, Yu XH, Lemaux PG, Cho MJ (2009) Stability and inheritance of endosperm-specific expression of two transgenes in progeny from crossing independently transformed barley plants. Plant Cell Rep 28:1265-1272   DOI
18 Clark DG, Dervinis C, Barrett JE, Klee H, Jones M (2004) Droughtinduced leaf senescence and horticultural performance of transgenic PSAG12-ipt petunias. J Amer Soc Hort SCI 129:93-99
19 Fang GC, Hanau RM, Vaillancourt LJ (2002) The SOD2 gene, encoding a manganese-type superoxide dismutase, is upregulated during conidiogenesis in the plant pathogenic fungus Colletotrichum graminicola. Fungal Genetics and Biology 36:155-165   DOI
20 François IEJA, Broekaert WF, Cammue BPA (2002) Different approaches for multi-transgene-stacking in plants. Plant Sci 163:281-295   DOI
21 Kim JS, Lee BH, Kwon SY, Kim YH, Kim SH, Cho KY (2005) Antioxidative responses of transgenic tobacco plants expressing both superoxide dismutase and ascorbate peroxidase in chloroplasts to several herbicides. Kor J Plant Biotech 32: 97-103   과학기술학회마을   DOI
22 Kim KU, Kim DU, Kwon ST (1986) Development of herbicide (paraquat) tolerant plant through tissue culture. Korea J Weed Sci 61:191-200
23 Kwon DY, Jeong YI, Lee HS, Kim JS, Cho KY, Allen RD, Kwak SS (2002) Enhanced tolerance of transgenic tobacco plants expressing both superoxide dismutase and ascorbate peroxidase in chloroplasts against methyl viologen-mediated oxidative stress. Plant, Cell and Environment 25:873-882   DOI
24 Chan YL, Lin KH, Sanjaya, Liao LJ, Chen WH, Chan MT (2005) Gene stacking in Phalenopsis orchid enhances dual tolerance to pathogen attack. Trans Res 14:279-288   DOI