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http://dx.doi.org/10.5010/JPB.2011.38.3.198

The use of cotyledonary-node explants in Agrobacterium tumefaciensmediated transformation of cucumber (Cucumis sativus L.)  

Jang, Hyun-A (Department of Oriental Pharmaceutical Development, Nambu University)
Kim, Hyun-A (Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Kwon, Suk-Yoon (Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Choi, Dong-Woog (Department of Biological Education, Jeonnam National University)
Choi, Pil-Son (Department of Oriental Pharmaceutical Development, Nambu University)
Publication Information
Journal of Plant Biotechnology / v.38, no.3, 2011 , pp. 198-202 More about this Journal
Abstract
Agrobacterium tumefaciens-mediated cotyledonary-node explants transformation was used to produce transgenic cucumber. Cotyledonary-node explants of cucumber (Cucumis sativus L. cv., Eunsung) were co-cultivated with Agrobacterium strains (EHA101) containing the binary vector (pPZP211) carrying with CaMV 35S promoter-nptII gene as selectable marker gene and 35S promoter-DQ gene (unpublished data) as target gene. The average of transformation efficiency (4.01%) was obtained from three times experiments and the maximum efficiency was shown at 5.97%. A total of 9 putative transgenic plants resistant to paromomycin were produced from the cultures of cotyledonary-node explants on selection medium. Among them, 6 transgenic plants showed that the nptII gene integrated into each genome of cucumber by Southern blot analysis.
Keywords
Agrobacterium; cotyledonary-node; paromomycin; transgenic cucumber;
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Times Cited By KSCI : 5  (Citation Analysis)
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