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http://dx.doi.org/10.5010/JPB.2011.38.2.186

Effects of ABA, reduced nitrogen source and osmoticum for somatic embryogenesis in Liriodendron tulipifera  

Kim, Yong-Wook (Division of Forest Biotechnology, Korea Forest Research Institute)
Han, Mu-Seok (Division of Forest Biotechnology, Korea Forest Research Institute)
Moon, Heung-Kyu (Division of Forest Biotechnology, Korea Forest Research Institute)
Park, So-Young (Division of Forest Biotechnology, Korea Forest Research Institute)
Publication Information
Journal of Plant Biotechnology / v.38, no.2, 2011 , pp. 186-190 More about this Journal
Abstract
This study was conducted to evaluate effects of various kinds or concentrations in abscisic acid (ABA), reduced nitrogen sources (casein hydrolysate, casamino acid and L-glutamine) and osmoticum for production of somatic embryos (SEs) from pro-embryogenic mass (PEM) in yellow poplar (Liriodendron tulipifera). In comparison of various concentrations of ABA, the highest number (640/10 mg PEM) of SEs was marked in the treatment of 0.5 mg/L. With higher concentration than 0.5 mg/L ABA, number of induced SEs were decreased. And the lowest number of SEs were obtained from the treatment of 20 mg/L ABA. Differences of 8 treatments of the nitrogen sources in the medium were also compared. In the experiment of 8 treatments for SEs production, the highest result showed in the treatment of 500 mg/L casamino acid (223/5 mg PEM). In comparison of different kinds/concentrations of osmotica for SEs induction, the best response was obtained from the treatment of 4% sucrose (317/5 mg PEM). In contrast, no SEs were found from the treatments supplemented with any concentrations of maltose.
Keywords
abscisic acid; nitrogen sources; osmotica; somatic embryo; yellow poplar;
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Times Cited By KSCI : 2  (Citation Analysis)
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