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http://dx.doi.org/10.5010/JPB.2009.36.4.403

Plant regeneration from callus derived root of northen type in garlic (Allium sativum L.)  

Ahn, Yul-Kyun (Vegetable Research Div., National Institute of Horticultural & Herbal Science, RDA)
Kim, Do-Sun (Vegetable Research Div., National Institute of Horticultural & Herbal Science, RDA)
Yoon, Moon-Kyoung (Vegetable Research Div., National Institute of Horticultural & Herbal Science, RDA)
Publication Information
Journal of Plant Biotechnology / v.36, no.4, 2009 , pp. 403-406 More about this Journal
Abstract
This study was conducted to develop an effective production of callus induction and plant regeneration system for garlic transformation. The best callus production occurred on in vitro root segment initially cultured on MS medium with 1.0 mg/L 2,4-D and 0.2 mg/L IAA in both ‘Danyang' and ‘Euseong'. The frequency of callus formation were 81.2% ‘Danyang' and 76.1% ‘Euseong'. Eight weeks after callus induction, callus lines were transferred to regeneration medium during 7 weeks. The best shoot regeneration medium was MS supplemented with 5 mg/L Kinetin and 1 mg/L NAA for ‘Danyang' and MS supplemented with 10 mg/L BAP for ‘Euseong'. The frequency of shoot regeneration were 51.5% ‘Danyang' and 56.6% ‘Euseong' The plantlets were acclimatized and transferred to the greenhouse with almost survival. This in vitro regeneration system should be useful for garlic transformation.
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1 Al Zahim MA, Ford Lloyd BV, Newbury HJ(1999) Detection of somaclonal variation in garlic (Allium sativum L.) using RAPD and cytological analysis. Plant Cell Rep 18:473-477   DOI   ScienceOn
2 Ali A, Metwally EE (1992) Somatic embryogenesis and plant regeneration as a tool for garlic improvement. Egypt J Appl Sci 7:727-735
3 Novak FJ (1990) Allium tissue culture. In: Rabinowitch HD&Brewster JL (eds) Onions and Allied Crops, Vol. 1 (pp 233-250). CRC, Boca Raton, Florida
4 Haque MS, Wada T, Hattori K (1997) High frequency shoot regeneration and plantlet formation from root tip of garlic. Plant Cell Tiss Organ Cult 50:83-89   DOI   ScienceOn
5 Wang HL, Kang YQ, Zhang CJ (1994) Embryogenesis via culture of garlic sprout leaf. Acta Agric Boreali Sin. 9:92-94
6 Barandiaran X, Martin N, Rodriguez Conde M, Di Pietro A, Martin J (1999a) Genetic variability in callus formation and regeneration of garlic (Allium sativum L.). Plant Cell Rep 18:434-437   DOI   ScienceOn
7 Chung HD, Chang MU (1979) Studies on infection of virus in garlic in Korea. J Kor Soc Hort Sci 20(2):123-129
8 Hwang JM, Kim JI, Oh SM, Uhm JS, Ha HT (2004) Field test of virus free seed garlic derived from tissue culture. J Kor Soc Hort Sci Techonol 22(4):411-415
9 Kehr AE, Schaeffer GW (1976) Tissue culture and differentiation of garlic. HortSci 11:422-423
10 Koch M, Tanami Z & Salomon R (1995) Improved regeneration of shoots from garlic callus. HortScience 30:378
11 Lot H, Delecolle B, Bocardo G, Mazachi C, Milne R. (1994) Partial charaterization of retrovirus-like particles associated with garlic dwarf disease. Plant Pathol 43:537-546   DOI   ScienceOn
12 Robledo PA, Villalobos Arambula VM, Jofre Garfias AE (2000) Efficient plant regeneration of garlic (Allium sativum L.) by root tip culture. In Vitro Cell Dev Biol Plant 36:416-419   DOI   ScienceOn
13 Nagasawa A, Finer JJ (1988) Induction of morphogenic callus cultures from leaf tissue of garlic. HortScience 23:1068-1070
14 Myers JM, Simon PW (1998) Continuous callus production and regeneration of garlic (Allium sativum L.) using root segments from shoot tip-derived plantlets. Plant Cell Rep 17:726-730   DOI   ScienceOn
15 Barandiaran X, Martin N, Rodriguez Conde M, Di Pietro A, Martin J (1999b) An efficient method for callus culture and shoot regeneration of garlic (Allium sativum L.). HortScience 34:348-349