Browse > Article
http://dx.doi.org/10.5010/JPB.2009.36.3.207

Current status on metabolic engineering of starch in sweetpotato  

Ahn, Young-Ock (Environmental Biotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Yang, Kyoung-Sil (Environmental Biotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Kim, Sun-Hyung (Department of Environmental Horticulture, University of Seoul)
Kwak, Sang-Soo (Environmental Biotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Lee, Haeng-Soon (Environmental Biotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Publication Information
Journal of Plant Biotechnology / v.36, no.3, 2009 , pp. 207-213 More about this Journal
Abstract
Starch serves not only as an energy source for plants, animals, and humans but also as an environmentally friendly alternative for fossil fuels. Progress in understanding of starch biosynthesis, and the isolation of many genes involved in this process have enabled the genetic modification of crops in a rational manner to produce novel starches with improved functionality. Starch is composed of two glucose polymers, amylose and amylopectin. The amylose and amylopectin ratio in starch affects its physical and physicochemical properties. Alteration in starch structure can be achieved by modifying genes encoding the enzymes responsible for starch biosynthesis and starch hydrolysis. Here, we describe recent findings concerning the starch modification in sweetpotato. Sweetpotato [Ipomoea batatas (L.) Lam] ranks seventh in annual production among food crops in the world as an important starch source. To develop transgenic sweetpotato plants with modifying starch composition, we constructed transformation vectors overexpressing granule bound starch synthase I and inhibiting amylopectin synthesis genes such as starch branching enzyme and isoamylase under the control of 35S promoter, respectively. Transformation of sweetpotato (cv. Yulmi) is in progress.
Keywords
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
연도 인용수 순위
1 Lin KH, Fu H, Chan CH, Lo HF, Shin MC, Chang YM, Chen LF (2008) Generation and analyses of the transgenic potatoes expressing heterologous thermostable β-amylase. Plant Sci 174:649-657   DOI   ScienceOn
2 Marshall J, Sidebottom C, Debet M, Martin C, Smith AM, Edwards A (1996) Identification of the major starch synthase in the soluble fraction of potato tubers. Plant Cell 8:1121-1135   DOI   ScienceOn
3 Morell MK, Kosar-Hashemi B, Cmiel M, Samuel MS, Chandler P, Raham S, Buleon A, Batey IL, Li Z (2003) Barley sex6 mutants lack starch synthase IIa activity and contain a starch with novel properties. Plant J 34:173-185   DOI   ScienceOn
4 Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473-497   DOI
5 Noda T, Kimura T, Otani M, Shimada T, Saito A, Suda I (2002) Physiochemical properties of amylose-free starch from transgenic sweetpotato. Carbohydr Polym 49:253-260   DOI   ScienceOn
6 Otani M, Hamada T, Katayama K, Kitahara K, Kim SH, Takahata Y, Suganuma T, Shimada T (2007) Inhibition of the gene expression for granule-bound starch synthase I by RNA interference in sweetpotato plants. Plant Cell Rep 26:1801-1807   DOI   ScienceOn
7 Jobling SA, Jarman C, Teh MM, Holmberg N, Blake C, Verhoeyen ME (2003) Immunomodulation of enzyme function in plants by single-domain antibody fragments. Nat Biotechnol 21: 77-80   DOI   ScienceOn
8 Schwall GP, SAfford R, Westcott RJ, Feffcoat R, Tayal A, Shi YC, Gidley MJ, Jobling SA (2000) Production of very-high- amylose potato starch by inhibition of SBE A and B. Nat Biotechnol 18:551-554   DOI   ScienceOn
9 Safford R, Jobling SA, Sidebottom CM, Westcott RJ, Cooke D, Tober KJ, Stronglitharm BH, Russell A, Gidley MJ (1998) Consequences of antisense RNA inhibition of starch branching enzyme activity on properties of potato starch. Carbohyd Polym 35:155-168   DOI   ScienceOn
10 Regierer B, Fernie AR, Springer F, Perez-Melis A, Leisse A, Koetl K, Willmitzer L, Geigenberger P, Kossmann J (2002) Starch conent and yield increase as a result of altering adenylate pools in transgenic plants. Nature Biotechnol 20:1256-1260   DOI   ScienceOn
11 Satoh H, Nishi A, Yamashita K, Takemoto Y, Tanaka Y, Hosaka Y, Sakurai A, Fujita N, Nakamura Y (2003) Starch branching enzyme -1-deficient mutation specifically affects the structure and properties of starch in rice endosperm. Plant Physiol 133: 1111-1121   DOI   ScienceOn
12 Smidansky ED, Clancy M, Meyer FD, Lanning SP, Blake NK, Talbert LE, Giroux MJ (2002) Enhanced ADP-glucose pyrophosphorylase activity in wheat endosperm increases seed yield. Proc Natl Acad Sci 9K, 1724-1729
13 Lim S, Kim YH, Kim SH, Kwon SY, Lee HS, Kim JS, Cho KY, Paek KY, Kwak SS (2007) Enhanced tolerance of transgenic sweetpotato plants that express both CuZnSOD and APX in chloroplasts to methyl viologen-mediated oxidative stress and chilling. Mol Breeding 19:227-239   DOI   ScienceOn
14 Hanashiro I, Itoh K, Kuratomi Y, Yamazaki M, Igarashi T, Matsugasako J, Takeda Y (2008) Granule-bound starch synthase I is responsible for biosynthesis of extra-long unit chains of amylopectin in rice. Plant Cell Physiol 49:925-933   DOI   ScienceOn
15 Tanaka M, Takahata Y, Nakayama H, Natatani M, Tahara M (2009) Altered carbohydrate metabolism in the storage roots gene, which encodes a Dof zinc finger transcription factor. Planta (Published online)
16 Torney F, Moeller L, Scarpa A, Wang K (2007) Genetic engineering approaches to improve bioethanol production from maize. 18: 193-199
17 Min SR, Bae JM, Harn CH, Jeong WJ, Lee YB, Liu JR (2007) Inhibition of starch biosynthesis by antisense expression of cDNAs encoding ADP-glucose pyrophosphorylase small subunit in sweetpotato. J Plant Biotechnol 34:277-283   DOI   ScienceOn
18 Visser RG, Somhorst I, Kuipers GJ, Ruys NJ, Feenstra WJ, Jacobsen E (1991) Inhibition of the expression of the gene for granule-bound starch synthase in potato by antisense constructs. Mol Gen Genet 225:289-296   DOI   ScienceOn
19 Jobling SA (2004) Improving starch for food and industrial applications. Current Opinion in Plant Biol 7:210-218   DOI   ScienceOn
20 Jobling SA, Westcott RJ, Tayal A, Jeffcoat R, Schwall G (2002) Production of a freeze-thaw-stable potato starch by antisense inhibition of three starch synthase genes. Nat Biotechnol 20:295-299   DOI   ScienceOn
21 Shimada T, Otani M, Hamada T, Kim SH (2006) Increase of amylose content of sweetpotato starch by RNA interference of the starch branching enzyme II gene (IbSBEII). Plant Biotechnol 23:85-90   DOI   ScienceOn
22 Wang Z, Chen X, Wang J, Liu T, Liu Y, Zhao L, Wang G (2007) Increasing maize seed weight by enhancing the cytoplasmic ADP-glucose pyrophosphorylase activity in transgenic maize plants. Plant Cell Tiss Organ Cult 88:83-92   DOI   ScienceOn
23 Edwards A, Fulton DC, Hylton CM, Jobling SA, Gidley M, Rossner U, Martin C, Smith AM (1999) A combined reduction in activity of starch synthase II and III of potato has novel effects on the starch of tubers. Plant J 17:251-261   DOI   ScienceOn
24 Chiang CM, YH FS, Huang LF, Tseng TH, Chung MC, Wang CS, Lur HS, Shaw JF, Yu SM (2005) Expression of a bi-functional and thermostable amylopullulanase in transgenic rice deeds leads to autohydrolysis and altered composition of starch. Mol Breeding 15:125-143   DOI   ScienceOn
25 Edwards A, Marshall J, Sidebottom C, Visser RG, Smith AM, Martin C (1995) Biochemical and molecular characterization of a novel starch synthase from potato tubers. Plant J 8: 283-294   DOI   ScienceOn
26 Itoh K, Ozaki H, Okada K, Hori H, Takeda Y, Mitsui T (2003) Introduction of Wx transgene into rice wx mutants leads to both high- and low- amylose rice. Plant Cell Physiol 44: 473-480   DOI   ScienceOn
27 Kim YH, Lim S, Yang KS, Kim CY, Kwon SY, Lee HS, Wang X, Zhou Z, Ma D, Yun DJ, Kwak SS (2009) Expression of Arabidopsis NDPK2 increases antioxidant activities and enhances tolerance to multiple environmental stresses in transgenic sweetpotato plants. Mol Breeding 24:233-244   DOI   ScienceOn
28 Lim S, Yang KS, Kwon SY, Paek KY, Kwak SS, Lee HS (2004) Agrobacterium-mediated genetic transformation and plant regeneration of sweetpotato (Ipomoea batatas). Korean J Plant Biotechnol 31:267-271   과학기술학회마을   DOI   ScienceOn
29 Jobling SA, Schwall GP, Westcott RJ, Sidebottom CM, Debet M, Gidley MJ, Jeffcoat R, Safford R (1999) A minor form of starch branching enzyme in potato (Solanum tuberosum) tubers has a major effect on starch structure: cloning and characterization of multiple forms of SBE A. Plant J 18: 163-171   DOI   ScienceOn
30 Kwon EJ, Kwon SY, Kim MZ, Lee JS, Ahn YS, Jeong BC, Kwak SS, Lee HS (2002) Plant regeneration of major cultivars of sweetpotato (Ipomoea batatas) in Korea via somatic embryogenesis. Korean J Plant Biotechnol 29: 189-192   과학기술학회마을   DOI   ScienceOn
31 Yang KS, Lim S, Kwon SY, Kwak SS, Kim HS, Lee HS (2005) Transgenic sweetpotato (Ipomoea batatas) expressing spike gene of porcine epidemic diarrhea virus. Korean J Plant Biotechnol 32:263-268   과학기술학회마을   DOI   ScienceOn
32 Kitahara K, Hamasuna K, Nozuma K, Otani M, Hamada T, Shimada T, Fujita K, Suganuma T (2007) Physiochemical properties of amylose-free and high-amylose starches from transgenic sweetpotato modified by RNA interference. Carbohydrate Polymers 69:233-240   DOI   ScienceOn
33 Kimura T, Otani M, Noda T, Ideta O, Hsimada T, Saito A (2001) Absence of amylose in sweetpotato [Ipomoea batatas (L.) Lam.] following the introduction of granule-bound starch synthase I cDNA. Plant Cell Rep 20:663-666   DOI