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http://dx.doi.org/10.5010/JPB.2007.34.4.307

Genetic Transformation of Chlamydomonas reinhardtii with the RNAi Suppressor p19 Gene of Tombus Virus  

Jeong, Won-Joong (Plant Genomics Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Liu, Jang-Ryol (Plant Genomics Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Cerutti, Heriberto (Biological Sciences and Plant Science Initiative, University of Nebraska)
Publication Information
Journal of Plant Biotechnology / v.34, no.4, 2007 , pp. 307-312 More about this Journal
Abstract
Chlamydomonas reinhardtii was transformed with the coding sequence of the Tombus virus gene p19 to determine whether the gene functions as an RNAi suppressor in C. reinhardtii. Transformants were confirmed to have 1 to several copies of p19 gene in their chromosomes. When an RNAi strain of C. reinhardtii generated by transforming the inverted repeat (IR) sequence homologous to the 3'UTR region of the MAA7 gene was re-transformed with the gene p19, MAA7 transcript levels of transformants fluctuated and proliferation of trans-formants on the medium containing 5-FI was suppressed. Overall results suggest that p19-mediated silencing suppression works at a low level in C. reinhardtii because of difference in codon usage resulting in weak P19 expression unless p19-mediated silencing suppression in C. reinhardtii works in a different manner from higher plants.
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