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http://dx.doi.org/10.5010/JPB.2007.34.4.277

Inhibition of Starch Biosynthesis by Antisense Expression of cDNAs Encoding ADP-Glucose Pyrophosphorylase Small Subunit in Sweetpotato  

Min, Sung-Ran (Plant Genome Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Bae, Jung-Myung (School of Life Sciences and Biotechnology, Korea University)
Harn, Chee-Hark (Biotechnology Institute, Nongwoo Bio Co., Ltd.)
Jeong, Won-Joong (Plant Genome Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Lee, Young-Bok (Dept. of Horticulture, Chungnam National University)
Liu, Jang-Ryol (Plant Genome Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Publication Information
Journal of Plant Biotechnology / v.34, no.4, 2007 , pp. 277-283 More about this Journal
Abstract
Embryogenic calluses derived from shoot apical meristem explants of sweetpotato were subjected to particle bombardment to generate transgenic plants for antisense expression of cDNAs encoding two different AGPase small subunit (ibAGP1 and ibAGP2). Plants were generated via somatic embryogenesis. PCR and Southern analysis demonstrated that the incorporation of ibAGP1 and ibAGP2 into the genome in an antisense orientation. Immunoblot analysis confirmed reduced levels of AGPase small subunit in transgenic plant leaves. Plants with both ibAGP1 and ibAGP2 produced a lower level of the protein than plants with ibAGP1 alone. iodine test demonstrated that transgenic plant leaves and storage root accumulated reduced amounts of starch. Iodine staining of leaf tissues indicated that transgenic plants accumulated less amount of starch than control. In accordance with western blot analysis, plants with both ibAGP1 and ibAGP2 accumulated a lower amount of starch than plants with ibAGP1 alone. Both transgenic plants exhibited a severely retarded growth, resulting in bare survival. It is suggested that disrupted expression of the gene encoding AGPase small subunit is lethal to the growth of sweetpotato contrast to other species including potato.
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