Browse > Article
http://dx.doi.org/10.5010/JPB.2006.33.2.123

The Expression of a Cytosolic Fructose-1,6-Bisphosphatase, a Key Enzyme in Sucrose Biosynthesis, Gene was Diurnally Fluctuated and Increased in Cold Acclimated Leaves of Chinese Cabbage  

Leen, Jeong-Yeo (Plant Genomic Institute, School of Bioscience and Biotechnology, Chungnam National University)
Song, Ha-Young (Plant Genomic Institute, School of Bioscience and Biotechnology, Chungnam National University)
Lim, Yong-Pyo (Plant Genomic Institute, School of Bioscience and Biotechnology, Chungnam National University)
Hur, Yoon-Kang (Plant Genomic Institute, School of Bioscience and Biotechnology, Chungnam National University)
Publication Information
Journal of Plant Biotechnology / v.33, no.2, 2006 , pp. 123-131 More about this Journal
Abstract
Chinese cabbage (Brassica rapa ssp. pekinesis) is one of the most important vegetable crops in korea and other East Asian countries. Cytosolic fructose-1,6-bisphospha-tase (cytFBPase) is a key enzyme in sucrose biosyn-thesis, which controls the sucrose levels as well as the productivity at plants. The Chinese cabbage cytFBPase gene, BrFBPase, encodes the 340 amino acid polypep-tide, giving a theoretical molecular weight of 37.2 kD and a isolectric point of 5.4. BrFBPase showed high sequence identity with Brassica homologs and its functional domains, such as 12,6P$_2$ binding site or active site and F6P binding site, were highly conserved in diverse sources of organisms. Although the genome of Chinese cabbage seemed to be triplicated, BrFBPase appears to be a single copy gene. The expression of BrFBPase was examined at transcript and protein levels under various conditions. BrFBPase expression was observed only in photosynthetic source tissue, not in sink tissue. The expression was slightly higher during the day than at night, and it showed a diurnal cycle with circadian rhythmicity. Short-term exposure to low temperature inhibited the expression of the BrFBPase, while long-term exposure increased the expression, supporting that sugar levels are high in late autumn when temperature are low.
Keywords
Chinese cabbage; BrFBPase; gene expression; low temperature; diurnal fluctuation; sugar content;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Daie J (1988) Mechanism of drought-induced alterations in assimilate partitioning and transport in crops. CRC Crit Rev Plant Sci 7: 117-137   DOI   ScienceOn
2 Goldschmidt EE, Huber SC (1992) Regulation of photosynthesis by end-product accumulation in leaves of plants storing starch, sucrose, and hexose sugars. Plant Physiol 99: 1443-1448   DOI   ScienceOn
3 Hodgson RJ, Jia Z, Plaxton WC (1998) A fluorescence study of ligand-induced conformational changes in cytosolic fructose-1,6-bisphosphatase from germinating castor oil seeds. Biochim Biophys Acta 1388: 285-294   DOI   ScienceOn
4 Holaday AS, Martindale W, Alred R, Brooks AL, Leegood RC (1992) Changes in activities of enzymes of carbon metabolism in leaves during exposure of plants to low temperature. Plant Physiol 98: 1105-1114   DOI   ScienceOn
5 Niittyla T, Messerli G, Trevisan M, Chen J, Smith AM, Zeeman SC (2004) A previously unknown maltose transporter essential for starch degradation in leaves. Science 303: 87-89   DOI   ScienceOn
6 Olien CR, Clark JL (1993) Changes in soluble carbohydrate composition of barley, wheat, and rye during winter. Crop Sci 85: 21-29
7 Pitcher LH, Daie J (1991) Growth and sink to source transition in developing leaves of sugar beet. Plant Cell Physiol 32: 335-342   DOI
8 Saghai-Maroof MA, Soliman KM, Jorgensen KA, Allard RW (1984) Ribosomal DNA spacer-length polymorphisms in barley: Mendelian inheritance, chromosomal location, and population dynamics. Proc Natl Acad Sci 81: 8014-8018
9 Sambrook J, Russell DW (2001) Molecular Cloning: A Laboratory Manual. 3rd ed., Cold Spring Harbor Press, Cold Spring Harbor, New York
10 Carpenter JF, Crowe JH (1988) The mechanism of cryoprotection of proteins by solutes. Cryobiology 25: 244-255   DOI   ScienceOn
11 Chueca A, Lazaro J, Gorge J (1984) Light-induced nuclear synthesis of spinach chloroplast fructose-1,6-bisphosphatase. Plant Physiol 75: 539-541   DOI   ScienceOn
12 Daie J (1993) Cytosolic fructose-1,6-bisphosphatase: A key enzyme in the sucrose biosynthetic pathway. Photosynth Res 38: 5-14   DOI   ScienceOn
13 Strand A, Hurry V, Gustafsson P, Gardestrom P (1997) Development of Arabidopsis thaliana leaves at low temperatures releases the suppression of photosynthesis and photosynthetic gene expression despite the accumulation of soluble carbohydrates. Plant J 12: 605-614   DOI   ScienceOn
14 Weiner H, McMichael RW, Huber SC (1992) Identification of factors regulating the phosphorylation status of sucrose- phosphate synthase in vivo. Plant Physiol 99: 1435-1442   DOI   ScienceOn
15 Weise SE, Kim KS, Stewart RP, Sharkey TD (2005) beta-Maltose is the metabolically active anomer of maltose during transitory starch degradation. Plant Physiol 137: 756-761   DOI   ScienceOn
16 Huber SC, Huber JL (1992) Role of sucrose-phosphate synthase in sucrose metabolism in leaves. Plant Physiol 99: 1275-1278   DOI   ScienceOn
17 Huber SC, Kerr PS, Torres WK (1985) Regulation of sucrose synthesis and movement. In: Heath RL, Preiss J (eds.), Regulation of Carbon Partitioning in Photosynthetic Tissue, Williams & Wilkins, Baltimore, MD, pp 199-214
18 Hur Y, Vasconcelos AC (1998) Spinach cytosolic fructose-1, 6-bisphosphatase: II. Light effect on its expression. Mol Cell 8: 148-156
19 Kelly GJ, Zimmerman G, Latzko E (1982) Fructose-bisphosphatase from spinach leaf chloroplast and cytoplasm. Methods Enzymol 90: 371-378   DOI
20 Koster KL, Lynch DV (1992) Solute accumulation and compartmentation during the cold acclimation of Puma rye. Plant Physiol 98: 108-113   DOI   ScienceOn
21 Lee SW, Hahn TR (2003) Light-regulated differential expression of pea chloroplast and cytosolic fructose-1,6bisphosphatases. Plant Cell Rep 21: 611-618
22 Nel W, Terblanche SE (1992) Plant fructose-1,6-bisphosphatase: Characteristics and properties. Int J Biochem 24: 1267-1283   DOI   ScienceOn
23 Schleucher J, Vanderveer PJ, Sharkey TD (1998) Export of carbon from the chloroplast at night. Plant Physiol 118: 1439-1445   DOI   ScienceOn
24 Schreiber U, Schliwa U, Bilger W (1986) Continuous recording of photochemical and non-photochemical chlorophyll fluorescence quenching with a new type of modulation fluorometer. Photosynth Res 10: 51-62   DOI   ScienceOn
25 Stitt M (1987). Limitation of photosynthesis by sucrose synthesis. In: Biggens J (Ed.), Progress in Photosynthesis Research, Vol. III, Martinus Nijhoff Publishers, pp. 685-692
26 Stitt M (1988) Control of sucrose synthesis. In: Barber J, Malkin R (eds.), Techniques and New Developments in Photosynthetic Research, Plenum, London, pp. 365-386
27 Anchordouguy T J, Rudolph AS, Carpenter JF, Crowe JH (1987) Mode of interaction of cryoprotectants with membrane phospholipids during freezing. Cryobiology 24: 324-331   DOI   ScienceOn
28 Stitt M (1990) The flux of carbon between the chloroplast and cytoplasm. In: Dennis DT, Trupin DH (Eds.), Plant Physiology, Biochemistry and Molecular Biology, Longman Scientific and Technical, New York, pp. 309-326
29 Stitt M, Grosse H (1988) Interactions between sucrose synthesis and $CO_2$ fixation IV. Temperature-dependent adjustment of the relation between sucrose synthesis and $CO_2$ fixation. J Plant Physiol 133: 392-400   DOI
30 Stitt M, Quick WP (1989) Photosynthetic carbon partitioning: its regulation and possibilities for manipulation. Physiol Plant 77: 633-641   DOI
31 Marcus F, Edelstein I, Reardon I, Heinrikson RL (1982) Complete amino acid sequence of pig kidney fructose1,6-bisphosphatase. Proc Natl Acad Sci 79: 7161-7165
32 Khayat E, Ham C, Daie J (1993) Purification and lightdependent molecular modulation of the cytosolic fructose1,6- bisphosphatase in sugar beet leaves. Plant Physiol 101: 57-64   DOI
33 Tognetti JA, Salerno GL, Crespi MD, Pontis HG (1990) Sucrose and fructan metabolism of different wheat cultivars at chilling temperatures. Physiol Plant 78: 554-559   DOI