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http://dx.doi.org/10.5010/JPB.2006.33.1.011

Isolation of SYP61/OSMl that is Required for Salt Tolerance in Arabidopsis by T-DNA Tagging  

Kim, Ji-Yeon (Division of Applied Life Science (BK21 program), and Environmental Biotechnology National Core Research Center, Gyeongsang National Univerity)
Baek, Dong-Won (Division of Applied Life Science (BK21 program), and Environmental Biotechnology National Core Research Center, Gyeongsang National Univerity)
Lee, Hyo-Jung (Division of Applied Life Science (BK21 program), and Environmental Biotechnology National Core Research Center, Gyeongsang National Univerity)
Shin, Dong-Jin (Division of Applied Life Science (BK21 program), and Environmental Biotechnology National Core Research Center, Gyeongsang National Univerity)
Lee, Ji-Young (Division of Applied Life Science (BK21 program), and Environmental Biotechnology National Core Research Center, Gyeongsang National Univerity)
Choi, Won-Kyun (Division of Applied Life Science (BK21 program), and Environmental Biotechnology National Core Research Center, Gyeongsang National Univerity)
Kim, Dong-Giun (Division of Applied Life Science (BK21 program), and Environmental Biotechnology National Core Research Center, Gyeongsang National Univerity)
Chung, Woo-Sik (Division of Applied Life Science (BK21 program), and Environmental Biotechnology National Core Research Center, Gyeongsang National Univerity)
Kwak, Sang-Soo (Environmental Biotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Yun, Dae-Jin (Division of Applied Life Science (BK21 program), and Environmental Biotechnology National Core Research Center, Gyeongsang National Univerity)
Publication Information
Journal of Plant Biotechnology / v.33, no.1, 2006 , pp. 11-18 More about this Journal
Abstract
Salt stress is one of major environmental factors influencing plant growth and development. To identify salt tolerance determinants in higher plants, a large-scale screen was conducted with a bialaphos marker-based T-DNA insertional collection of Arabidopsis ecotype C24 mutants. One line for salt stress-sensitive mutant (referred to as ssm1) exhibited increased sensitivity to both ionic (NaCl) and nonionic (mannitol) osmotic stress in a root growth assay. This result suggests that ssm1 mutant is involved in ion homeostasis and osmotic compensation in plant. Molecular cloning of the genomic DNA flanking T-DNA insert of ssm1 mutant was achieved by mutant genomic DNA library screening. T-DNA insertion appeared in the first exon of an open reading frame on F3M18.7, which is the same as AtSYP61. SSM1 is SYP61/OSM1 that is a member of the SNARE superfamily of proteins required for vesicular/target membrane fusions and factor related to abiotic stress.
Keywords
Genomic DNA library screening; ion homeostasis; osmotic compensation; salt stress; $ssm(salt\; \b{s}tress-\b{s}ensitive\; \b{M}utant)$;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
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