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http://dx.doi.org/10.5010/JPB.2005.32.4.269

Expression and Purification of Toll-like Receptor 9 Cytoplasmic Domain in Pichia patoris  

Lee Kyun-Young (Division of Applied Life Science, PMBBRC & EB-NCRC, Gyeongsang National University)
Lee Kon-Ho (Division of Applied Life Science, PMBBRC & EB-NCRC, Gyeongsang National University)
Publication Information
Journal of Plant Biotechnology / v.32, no.4, 2005 , pp. 269-273 More about this Journal
Abstract
Toll-like receptors (TLR) are important components of innate immunity in the defense against pathogens. TLRs recognize pathogen-associated common molecular patterns. TLRs are similar to the receptors involved in defense responses in plants. TLR protein is a type 1 membrane protein, consisting of an extracellular domain containing leucine-rich repeats and a cytoplasmic domain. The cytoplasmic domain delivers ligand recognition signals that result in production of anti-microbial agents. The cytoplasmic domain (amino acid 858-1032) of toll-like receptor 9 has been expressed using methylotrophic yeast Pichia pastoris. The protein expression was confirmed by Western-blot, N-terminal sequencing and MALDl-TOF mass spectrometry. The proteins have been purified by nickel affinity, cation exchange and gel-filtration chromatography.
Keywords
Pichia pastoris; Protein expression; protein purification; toll-like receptor 9 cytoplasmic domain;
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