Browse > Article
http://dx.doi.org/10.5010/JPB.2005.32.4.251

Proteomic Analysis of Cytokinin Induced Proteins in Arabidopsis  

Liang Ying-Shi (Division of Applied Life Science, Gyeongsang National University, PMBBRC, Gyeongsang National University)
Cha Joon-Yung (Division of Applied Life Science, Gyeongsang National University, PMBBRC, Gyeongsang National University, EBNCRC, Gyeongsang National University)
Ermawati Netty (Division of Applied Life Science, Gyeongsang National University, PMBBRC, Gyeongsang National University)
Jung Min-Hee (Division of Applied Life Science, Gyeongsang National University, PMBBRC, Gyeongsang National University)
Bae Dong-Won (Central Laboratory, Gyeongsang National University)
Lee Chang-Won (Department of Microbiology, Gyeongsang National University)
Son Dae-Young (Division of Applied Life Science, Gyeongsang National University, PMBBRC, Gyeongsang National University, EBNCRC, Gyeongsang National University)
Publication Information
Journal of Plant Biotechnology / v.32, no.4, 2005 , pp. 251-256 More about this Journal
Abstract
Cytokinins are essential plant hormones that play crucial roles in various aspects of plant growth and development. To better understand the molecular mechanisms of cytokinin action, we identified cytokinin related proteins by a proteomic approach. Proteins extracted from control and trans-zeatin treated Arabidopsis seedlings were separated and analyzed by two dimensional gel analysis. Differentially expressed protein spots were identified with peptide mass fingerprinting based on matrix assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry and database searching, We obtained ten up-regulated and one down-regulated proteins upon t-zeatin treatment. The expression of the following proteins was induced; pollen allergen like protein, L-ascorbate peroxidase, tetrapyrrole methylase family protein, SGT1 protein homolog, disease resistance related protein, maternal embryogenesis control protein, paxneb related protein, gluthathione S-transferase and IAA amino acid hydrolase homolog.
Keywords
MALDI-TOF mass spectrometry; trans-zeatin; two-dimentional gel electrophoresis;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Lu JL, Ertl JR, Chen CM (1990) Cytokinin enhancement of the light incubation of nitrate reductase transcript levels in etiolated barley leaves. Plant Mol Biol 14: 585-594   DOI
2 Menges M, Hennig L, Gruissem W, Murray JA (2002) Cell cycle regulated gene expression in Arabidopsis. J Biol Chem 277: 41987-42002   DOI   ScienceOn
3 Mok DW, Mok MC (2001) Cytokinin metabolism and action. Annu Rev Plant Physiol Plant Mol Biol 52: 89-118   DOI
4 Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol Plant 15: 473-497   DOI
5 Muskett P, Parker J (2003) Role of SGT1 in the regulation of plant R gene signalling. Microbes Infect 5: 969-976   DOI   ScienceOn
6 O’Farrell, PH (1975) High resolution two-dimensional electrophoresis of protein. J Biol Chem 250: 4007-4021
7 Rashotte AM, Carson SD, To JP, Kieber JJ (2003) Expression profiling of cytokinin action in Arabidopsis. Plant Physiol 132: 1998-2011   DOI
8 Rupp HM, Frank M, Werner T, Strnad M, Schmulling T (1999) Increased steady state mRNA levels of the STM and KNATI homeobox genes in cytokinin overproducing Arabidopsis thaliana indicate a role for cytokinins in the shoot apical meristem. Plant J 18: 557-563   DOI   ScienceOn
9 Suzuki I, Cretin C, Omata T, Sugiyama T (1994) Transcriptional and posttranscriptional regulation of nitrogen-responding expression of phosphoenol-pyurvate carboxylase gene in maize. Plant Physiol 105: 1223-1229   DOI
10 Flores S, Tobin EM (1989) Cytokinin modulation of LHCP mRNA levels: the involvement of post-transcriptional regulation. Plant Mol Biol 11: 409-415   DOI   ScienceOn
11 Howell SH, Lall S, Che P (2003) Cytokinins and shoot development. Trends Plant Sci 8: 453-459   DOI   ScienceOn
12 Jensen ON, Wilm M, Shevchenko A, Mann M. (1999) Sample preparation methods for mass spectrometric peptide mapping directly from 2-DE gels. Methods Mol Biol 112: 513-530
13 Kakimoto T (1996) CKI1, a histidine kinase homolog implicated in cytokinin signal transduction. Science 274: 982-985   DOI   ScienceOn
14 Kilili KG, Atanassova N, Vardanyan A, Clatot N, Al-Sabarna K, Kanellopoulos PN, Makris AM, Kampranis SC (2004) Differential roles of tau class glutathione S-transferases in oxidative stress. J Biol Chem 279: 24540-24551   DOI   ScienceOn
15 Longo GP, Bracale M, Rossi G, Longo CP (1990) Benzyladenine induces the appearance of LHCP-mRNA and of the relevant protein in dark-grown excised watermelon cotyledons. Plant Mol Biol 14: 569-573   DOI
16 Kim ST, Cho KS, Jang YS, Kang KY (2001) Two-dimensional electrophoretic analysis of rice proteins by polyethylene glycol fractionation for protein arrays. Electrophoresis 22: 2103-2109   DOI   ScienceOn
17 Landry F, Lombardo CR, Smith JW (2000) A method for application of samples to matrix-assisted laser desorption ionization time-of-flight targets that enhances peptide detection. Anal Biochem 279: 1-8   DOI   ScienceOn
18 Lebs S, Lebs W, Klyachko NL, Romanko EG, Kulaeva ON, Wollgiehn R, Parthier B (1984) Gene expression in cytokinin- and light-mediated plastogenesis of cucurbita cotylendons: ribulose-l ,5-bisphosphate carboxylase/oxygenase. Planta 162: 289-298   DOI   ScienceOn
19 Bianchi MW, Roux C, Vartanian N (2002) Drought regulation of GST8, encoding the Arabidopsis homologue of ParC/Nt107 glutathione transferase/peroxidase. Physiol Plant 116: 96-105   DOI   ScienceOn
20 Binns AN (1994) Cytokinin accumulation and action: biochemical, genetic and molecular approaches. Annnu Rev Plant Physiol Plant Mol Biol 45: 197-209   DOI
21 Blum H, Beier H, Gross HJ (1987) Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels. Electrophoresis 8: 93-99   DOI
22 Durmus N, Kadioglu A (2005) Reduction of paraquat toxicity in maize leaves by benzyladenine. Acta Biol Hung 56: 97-107   DOI   ScienceOn
23 Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248-254   DOI   ScienceOn
24 Crowell DN (1994) Cytokinin regulation of a soybean pollen allergen gene. Plant Mol Biol 25: 829-835   DOI