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http://dx.doi.org/10.5010/JPB.2005.32.3.201

Effect of BA and NAA on Adventitious Bud Induction From In Vitro Germinant Eucalyptus pellita  

Kim Ji-Ah (Division of Biotechnology, Korea Forest Research Institute (KFRI))
Moon Heung-Kyu (Division of Biotechnology, Korea Forest Research Institute (KFRI))
Kang Ho-Duck (Department of Forestry Resources, Dongguk University)
Publication Information
Journal of Plant Biotechnology / v.32, no.3, 2005 , pp. 201-207 More about this Journal
Abstract
This study was conducted to examine the effect of BA and NAA on adventitious bud induction from in vitro germinants E. pellita. The capacity of adventitious bud formation greatly depends on juvenility and explants origin; the more juvenile materials are the better ability to form adventitious buds even in in vitro raised plantlets. In case of in vitro germinants, 7 day old plantlets showed a better morphological response than did 14 day old ones in the induction of adventitious buds. The capacity to show morphological response was in decreasing order : cotyledons> petioles> roots. Ho adventitious buds formed when root segments were used as culture material. And optimum medium appeared to be MS + 0.5 mg/L BA and 0.2 mg/L NAA. Adventitious buds could be developed into multiple shoots and regenerated normal plantlets on DKW medium plus 0.2 mg/L BA and 0.01 mg/L NAA.
Keywords
Explant type; juvenility; plantlet formation;
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