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http://dx.doi.org/10.5010/JPB.2005.32.2.091

Production of Transgenic Maize (Zea mays L.) Using Agrobacterium tumefaciens-Mediated Transformation  

Cho Mi-Ae (Eugentech Inc)
Park Yun-Ok (Eugentech Inc)
Kim Jin-Suck (Korea Research Institute of Chemical Technology)
Park Ki-Jin (Maize Experiment Station, Gangwon-do Provincial Agricultural Research and Extension Service)
Min Hwang-Ki (Maize Experiment Station, Gangwon-do Provincial Agricultural Research and Extension Service)
Liu Jang-Ryol (Plant Cell Biotechnology Laboratory, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Clemente Tom (Plant Science Initiative, University of Nebraska-Lincoln)
Choi Pil-Son (Department of Medicinal Plant Resources, Nambu University)
Publication Information
Journal of Plant Biotechnology / v.32, no.2, 2005 , pp. 91-95 More about this Journal
Abstract
Agrobacterium tumefaciens-mediated immature embryo transformation was used to produce transgenic maize. Immature embryo of Hi II genotype were co-cultivated with strains Agrobacterium tumefaciens (C58C1) containing the binary vectors (pPTN290) carrying with Ubiquitin promoter-GUS gene as reporter gene and NOS promoter-nptll gene conferring resistance to paromomycin as selective agent. Seven embryogenic callus lines transformed showed the resistance in paromomycin antibiotics. Histochemical GUS assay showed that 7 individual lines transformed with the GUS gene were positive response among the transformants. Southern blot analysis revealed that the nptll gene segregated and expressed in their progeny.
Keywords
Agrobacterium strains; ${\beta}$-glucuroinidase (GUS); maize; transformation;
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