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http://dx.doi.org/10.5010/JPB.2004.31.4.255

Development of Transgenic Soybean Using Agrobacterium tumefaciens  

Cho, Mi-Ae (㈜유진텍 부설연구소)
Choi, Dong-Woog (㈜유진텍 부설연구소)
Liu, Jang-Ryol (한국생명공학연구원)
Clemente Tom (네브라스카대학)
Choi, Pil-Son (㈜유진텍 부설연구소, 남부대학교 생약자원학과)
Publication Information
Journal of Plant Biotechnology / v.31, no.4, 2004 , pp. 255-259 More about this Journal
Abstract
Agrobacterium tumefaciens-mediated cotyledonary node transformation was used to produce transgenic soybean. Cotyledonary node explants of three cultivars and one genotype were co-cultivated with strains Agrobacterium (LBA4404, GV3101, EHA101, C58) containing the binary vectors (pCAMBIA3301 and pPTN289) carrying with CaMV 35S promoter-GUS gene as reporter gene and NOS promoter-bar gene conferring resistance to glufosinate (herbicide Basta) as selectable marker. There was a significant difference in the transformation frequency depend on bacteria strain. The EHA101 strain of the bacterial strains employed gave the maximum efficiency (3.6%). One hundred-six lines transformed showed the resistance in glufosinate. Histochemical GUS assay showed that at least 11 plants transformed with the GUS gene were positive response. The soybean transformants were obtained from the Thorne (5 plants), 1049 (5 plants) and Bakun (1 plant), respectively. Southern blot analysis and leaf painting assay revealed that the GUS and bar gene segregated and expressed in their progeny.
Keywords
Agrobacterium strains; $\beta$-glucuoinidase (GUS); Glycine max L.; transformation;
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