Browse > Article
http://dx.doi.org/10.5010/JPB.2004.31.3.231

Astaxanthin Biosynthesis in Transgenic Arabidopsis by Using Chyb Gene Encoding β-Carotene Hydroxylase  

Lee, Ho-Jae (동의공업대학 식품생명과학과)
Kang, Kwon-Kyoo (한경대학교 원예학과)
Publication Information
Journal of Plant Biotechnology / v.31, no.3, 2004 , pp. 231-237 More about this Journal
Abstract
Oxycarotenoids are oxygenated carotenoids that perform critical roles in plants. $\beta$-Carotene hydroxylase adds hydroxyl groups to the $\beta$-rings of carotenes and has been cloned from several bacteria and plants including Arabidopsis. This study was carried out to investigate the effect of $\beta$-carotene hydroxylase gene (Chyb) on the oxycarotenoids biosynthesis in the transgenic Arabidopsis. Construct of pGCHYB containing Chyb was established onto Gateway vector system (pENTR3C gateway vector and pH2GW7 destination vector). Arabidopsis thaliana (cv. Columbia) was transformed with Agrobacterium tumerfacience GV3101 harboring pGCHYB construct driven by 35S promoter and hygromycin resistant gene. Seven hundred bases paired PCR products, indicating the presence of Chyb gene, were found in the transformants by PCR analysis using Chyb primers. Hygromycin resistance assay showed that transgenes were stably inherited to next generation. The overexpression of the Chyb gene resulted in the decrease carotenoid content. Especially, astaxanthin unusual oxycarotenoid in wild type Arabidopsis was detected in the transgenic plants. This means that decreased carotenoids might be converted into astaxanthin metabolism with the aid of silent gene in the host.
Keywords
Arabidopsis thaliana; astaxanthin; $\beta$-carotene hydroxylase; oxycarotenoid;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Fraser PD, Pinto ME, Holloway DE, Bramley PM (2000) Technical advance: application of high-performance liquid chromatography with photodiode array detection to the metabolic profiling of plant isoprenoids. Plant J 24: 551-558   DOI   ScienceOn
2 Gale CR, Hall NF, Phillips DI, Martyn CN (2003) Lutein and zeaxanthin status and risk of age-related macular degeneration. Invest Ophthalmol Vis Sci 44: 2461-2465   DOI   ScienceOn
3 Gallagher CE, Cervantes-Cervantes M, Wurtzel ET (2003) Surrogate biochemistry: use of Escherichia coli to identify plant cDNAs that impact metabolic engineering of carotenoid accumulation. Appl Microbiol Biotechnol 60: 713-719   DOI   ScienceOn
4 Kang B, Park Y (2001) Effect of antibiotics and herbicide on shoot regeneration from cotyledon and hypocotyl explants of chinese cabbage. Kor J Hort Sci Technol 19: 17-21
5 Kim Y, Soh W, Suh M, Hong C (1993) Transformation of Tobacco(Nicotiana tabacum) for Hygromycin-resistance. Korean J. Plant Tiss Cult 20: 103-107
6 Kotake-Nara E, Kushiro M, Zhang H, Sugawara T, Miyashita K, Nagao A (2001) Carotenoids affect proliferation of human prostate cancer cells. J Nutr 131: 3303-3306   DOI
7 Ladygin VG (2000) Biosynthesis of carotenoids in plastids of plants. Biochemistry (Mosc) 65: 1113-1128
8 Bouvier F, Keller Y, d'Harlingue A, Camara B (1998) Xanthophyll biosynthesis: molecular and functional characterization of carotenoid hydroxylases from pepper fruits (Capsicum annuum L.). Biochim Biophys Acta 1391: 320-328   DOI   ScienceOn
9 Breitenbach J, Misawa N, Kajiwara S, Sandmann G (1996) Expression in Escherichia coli and properties of the carotene ketolase from Haematococcus pluvialis. FEMS Microbiol Lett 140: 241-246   DOI
10 Clough SJ, Bent AF (1998) Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J 16: 735-743   DOI   ScienceOn
11 Curran-Celentano J, Hammond BR, Jr., Ciulla TA, Cooper DA, Pratt LM, Danis RB (2001) Relation between dietary intake, serum concentrations, and retinal concentrations of lutein and zeaxanthin in adults in a Midwest population. Am J Clin Nutr 74: 796-802   DOI
12 Davison PA, Hunter CN, Horton P (2002) Overexpression of beta-carotene hydroxylase enhances stress tolerance in Arabidopsis. Nature 418: 203-2066   DOI   ScienceOn
13 Gann PH, Ma J, Giovannucci E, Willett W, Sacks FM, Hennekens CH, Stampfer MJ (1999) Lower prostate cancer risk in men with elevated plasma lycopene levels: results of a prospective analysis. Cancer Res 59: 1225-1230
14 Nishino H, Murakosh M, Ii T, Takemura M, Kuchide M, Kanazawa M, Mou XY, Wada S, Masuda M, Ohsaka Y, Yogosawa S, Satomi Y, Jinno K (2002) Carotenoids in cancer chemoprevention. Cancer Metastasis Rev 21: 257-264   DOI   ScienceOn
15 Sandmann G (2002) Combinatorial biosynthesis of carotenoids in a heterologous host: a powerful approach for the biosynthesis of novel structures. Chembiochem 3: 629-635   DOI   ScienceOn
16 Shewmaker CK, Sheehy JA, Daley M, Colburn S, Ke DY (1999) Seed-specific overexpression of phytoene synthase: increase in carotenoids and other metabolic effects. Plant J 20: 401-412   DOI   ScienceOn
17 Giovannucci E (1999) Tomatoes, tomato-based products, lycopene, and cancer: review of the epidemiologic literature. J Natl Cancer Inst 91: 317-331   DOI   ScienceOn
18 Giuliano G, Aquilani R, Dharmapuri S (2000) Metabolic engineering of plant carotenoids. Trends Plant Sci 5: 406-409   DOI   ScienceOn
19 Gotz T, Sandmann G, Romer S (2002) Expression of a bacterial carotene hydroxylase gene (crtZ) enhances UV tolerance in tobacco. Plant Mol Biol 50: 129-142
20 Harker M, Hirschberg J (1997) Biosynthesis of ketocarotenoids in transgenic cyanobacteria expressing the algal gene for beta-C-4-oxygenase, crtO. FEBS Lett 404: 129-134   DOI   ScienceOn
21 Hirschberg J (2001) Carotenoid biosynthesis in flowering plants. Curr Opin Plant Biol 4: 210-218   DOI   ScienceOn
22 Miura Y, Kondo K, Saito T, Shimada H, Fraser PD, Misawa N (1998) Production of the carotenoids lycopene, beta-carotene, and astaxanthin in the food yeast Candida utilis. Appl Environ Microbiol 64: 1226-1229
23 Lotan T, Hirschberg J (1995) Cloning and expression in Escherichia coli of the gene encoding beta-C-4-oxygenase, that converts beta-carotene to the ketocarotenoid canthaxanthin in Haematococcus pluvialis. FEBS Lett 364: 125-128   DOI   ScienceOn
24 Mann V, Harker M, Pecker I, Hirschberg J (2000) Metabolic engineering of astaxanthin production in tobacco flowers. Nat Biotechnol 18: 888-892   DOI   ScienceOn
25 Mares-Perlman JA, Millen AE, Ficek TL, Hankinson SE (2002) The body of evidence to support a protective role for lutein and zeaxanthin in delaying chronic disease. Overview. J Nutr 132: 518S-524S   DOI
26 Murashige T, Sckoog F (1962) A revise medium for rapid growth and bioassays with tobacco tissues. Physiol Plant 15: 473-497   DOI
27 Tian L, Magallanes-Lundback M, Musetti V, DellaPenna D (2003) Functional analysis of beta- and epsilon-ring carotenoid hydroxylases in Arabidopsis. Plant Cell 15: 1320-1332   DOI   ScienceOn
28 Steinbrenner J, Linden H (2001) Regulation of two carotenoid biosynthesis genes coding for phytoene synthase and carotenoid hydroxylase during stress-induced astaxanthin formation in the green alga Haematococcus pluvialis. Plant Physiol 125: 810-817   DOI   ScienceOn
29 Sun Z, Gantt E, Cunningham FX, Jr. (1996) Cloning and functional analysis of the beta-carotene hydroxylase of Arabidopsis thaliana. J Biol Chem 271: 24349-24352   DOI   ScienceOn
30 Tian L, DellaPenna D (2001) Characterization of a second carotenoid beta-hydroxylase gene from Arabidopsis and its relationship to the LUT1 locus. Plant Mol Biol 47: 379-388   DOI   ScienceOn
31 Bone RA, Landrum JT, Guerra LH, Ruiz CA (2003) Lutein and zeaxanthin dietary supplements raise macular pigment density and serum concentrations of these carotenoids in humans. J Nutr 133: 992-998   DOI