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http://dx.doi.org/10.5010/JPB.2004.31.2.175

Expression of a Human Histone H1.5 in Transgenic Tobacco Cultured Cells  

Kim, Kee-Yeun (삼양제넥스 생명공학연구소)
Kwon, Suk-Yoon (한국생명공학연구원 환경생명공학연구실)
Song, Jae-Young (삼양제넥스 생명공학연구소)
Lee, Haeng-Soon (한국생명공학연구원 식물세포공학연구실)
Kwak, Sang-Soo (한국생명공학연구원 환경생명공학연구실)
Publication Information
Journal of Plant Biotechnology / v.31, no.2, 2004 , pp. 175-178 More about this Journal
Abstract
Transgenic tobacco (Nicotiana tabacum L. cv Bright Yellow-2) cell lines expressing a human histone H1.5 (referred to as hH1.5), which suppress collagen-induced rheumatoid arthritis, were developed under the oxidative stress-inducible peroxidase (SWPA2) promoter. Tobacco BY-2 cells were transformed by Agrobacterium-mediated method. The kanamycin-resistant calli were selected on the modified MS medium containing 150mg/L kanamycin and 300mg/L claforan. Transgenic cell lines were confirmed by PCR and northern blot analysis. Recombinant hH1.5 (rhH1.5) protein (42 kDa) was also detected by Western blot analysis, showing a different molecular weight of human hH1.5 (32 kDa). These results suggested that a hH1.5 gene was properly introduced in tobacco cultured cells under the control of SWPA2 promoter. The further characterization of rhH1.5 protein remains to be studied.
Keywords
Collagen-induced rheumatoid arthritis; cultured cells; histone H1.5; oxidative stress-inducible SWPA2 promoter;
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