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http://dx.doi.org/10.5010/JPB.2004.31.2.145

Anatomical Characteristics of Hyperhydric Shoots Occuring in In Vitro Culture of Peace Poplar  

Kang, Hyo-Jin (국립산림과학원 생물공학과)
Moon, Heung-Kyu (국립산림과학원 생물공학과)
Park, So-Young (국립산림과학원 생물공학과)
Kim, Pan-Gi (서울대학교 기초과학연구원)
Publication Information
Journal of Plant Biotechnology / v.31, no.2, 2004 , pp. 145-149 More about this Journal
Abstract
We investigated the anatomical aspects of vitrification in peace poplar. Comparisons were made with regard to characteristics occurring between hyperhydric and normal shoots in shoot proliferation cultures on MS medium containing 0.2 mg/L BA. Compared with normal plants, hyperhydric plants had thick, curled, and dark green leaves. Hyperhydric stems were thicker and shorter than those of normal stems. When examined under the microscopes, the mesophyll palisade cells of hyperhydric leaves were vacuolated, whereas those of normal leaves contained normal and enriched vacuole with cytoplasm. Generally, the hyperhydric leaves showed poorly developed palisade parenchyma, and revealed irregular and bigger sized intercellular structures in both palisade and spongy parenchyma as well as epidermis cells compare to those of normal leaves. In addition, the hyperhydric leaves had lower stomatal density and bigger sized cell. Vascular tissues of hyperhydic stems were less differentiated because of poorly lignified xylem tissue. The greatly expanded cortical cells and pith appeared to be the main cause of thick stems as compared with normal stems.
Keywords
Histology; hyperhydricity; P. koreana X P. trichocarpa; stomata;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
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