Browse > Article
http://dx.doi.org/10.5010/JPB.2004.31.1.083

Induction of a Sweetpotato Anion Peroxidase swpa2 Gene Expression by Stress-related Chemicals and Pectobacterium chrysanthemi  

Kim, Yun-Hee (Laboratory of Environmental Biotechnology, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Ryu, Sun-Hwa (Laboratory of Environmental Biotechnology, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Kim, Kee-Yeun (Laboratory of Environmental Biotechnology, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Kwon, Suk-Yoon (Laboratory of Environmental Biotechnology, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Bang, Jae-Wook (Department of Biology, Chungnam National University)
Kwak, Sang-Soo (Laboratory of Environmental Biotechnology, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Publication Information
Journal of Plant Biotechnology / v.31, no.1, 2004 , pp. 83-88 More about this Journal
Abstract
Expression of an anionic peroxidase swpa2 gene isolated from cultured cells of sweetpotato (Ipomoea batatas) was investigated under various stress conditions by RT-PCR. The swpa2 gene was not expressed in any tissues of intact sweetpotato plant grown at the normal condition. The expression of this gene was strongly induced in leaf tissue by treatment of $H_2O$$_2$ (440mM). Treatment of NaCl (100mM), ABA (0.1mM) and methyl jasmonate(MeJA, 0.1mM) also induced the expression of swpa2 gene. Interestingly, salicylic acid (SA, 0.1 mM) did not induce the expression of swpa2 gene, indicating that anionic swpa2 POD is differently involved in SA and MeJA signaling pathways. In addition, swpa2 gene was strongly induced in sweetpoato leaf tissues infected with Pectobacterium chrysanthemi, indicating that swpa2 is involved in defense related to the pathogenesis of P. chrysanthemi in sweetpotato plants. These results strongly suggest that swpa2 gene is involved in overcoming oxidative stresses caused by both abiotic and biotic stress.
Keywords
Peroxidase; sweetpotato; stress-related chemicals; oxidative stress; Pectobacterium chrysanthemi;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Baga M, Chibbar RN, Kartha KK (1995) Molecular cloning and expression analysis of peroxidase genes from wheat. Plant Mol Biol 29:647-662   DOI   ScienceOn
2 Choi SM, Lee OS, Kwon SY, Kwak SS, Yu DY, Lee HS (2003) High expression of a human lactoferrin in transgenic tobacco cell cultures. Biotech Lett 25: 213-218   DOI   ScienceOn
3 dark CA, Moyer JW (1988) Compendium of sweetpotato disease APS Press, St Paul. pp 74 truncatula precedes infection by Rhizobium meliloti. Plant Cell 7: 43-55   DOI   ScienceOn
4 Curtis MD, Rae AL, Rusu AG, Hanison SJ, Manners JM (1997) A peroxidase gene promoter induced by phytopathogens and methyl jasmonate in transgenic plants. Mol Plant Microb Interact 3: 326-338
5 Doares SH, Narvaez-Vasquez J, Conconin A, Ryan CA (1995) Salicylic acid inhibits synthesis of proteinase inhibitors in tomato leaves induced by systemin and jasmonic acid. Plant Physiol 108: 1741-1746
6 Felton GW, Donato K, Delvecchio RJ, Duffey SS (1989) Activation of plant foliar oxidase by insect feeding reduces nutritive quality of foliage for noctuid herbivores. J Chem Ecol 15: 2667-2694   DOI   ScienceOn
7 Gazarian IG, Lagrimini LM, Gillian AA, Thorneley NF (1996) Mechanism of indole-3-acetic acid oxidation by plant peroxidase: anaerobic stopped-flow spectrophotometric studies on horseradish and tobacco peroxidases. Biochem J 313: 841-847
8 Hiraga S, Yamamoto K, Ito H, Sasaki K, Matsui H, Honma M, Nagamura Y, Sasaki T, Ohashi Y (2000) Diverse expression profiles of 21 rice peroxidase genes. FEBS Lett 471: 245-250   DOI   ScienceOn
9 Kwon SY, Choi SM, Lee OS, Kwak SS, Lee HS (2003) Transgenic ginseng cell lines that produce high levels of a human lactofemn. Planta Med 69:1005-1008   DOI   ScienceOn
10 Lagnrnini LM (1996) The role of the tobacco of anionic peroxidase in growth and development, In: Obinger C, Bumer U, Ebermann R, Penel C, Greppin H, (eds), Plant Peroxidase: Biochemistry and Physiology, University of Agriculture, Vienna and University of Geneva, pp 235-242
11 Linsmaier EM, Skoog F (1965) Organic growth factor requirements of tobacco tissue cultures. Physiol Plant 18: 100-127   DOI
12 Mader M, Fussl DF (1982) Role of peroxidase in lignification of tobacco cells. Plant Physiol 70:1132-1134   DOI   ScienceOn
13 Naqvi SM, Park KS, Yi SY, Lee HW, Bok SH, Choi DI (1998) A glycine-rich RNA-binding protein gene is differentially expressed during acute hypersensitive response following tobacco mosaic virus infection in tobacco. Plant Mol Biol 37: 571-576   DOI   ScienceOn
14 Neil S, Desikan R, Hancock J (2002) Hydrogen peroxide signalling. Curr Opin Plant Biol 5: 388-395   DOI   ScienceOn
15 Park SY, Ryu SH, Kwon SY, Lee HS, Kim JG, Kwak SS (2003a) Molecular cloning and characterization of six peroxidase cDNAs from cell cultures of sweetpotato and their differential expressions in response to stress. Mol Genet Genomics 269: 542-552   DOI   ScienceOn
16 Park SY, Ryu SH, Kwon SY, Kim JG, Kwak SS (2003b) Molecular cloning and characterization of a peroxiredoxin cDNA from cell cultures of sweetpotato. Korean J Plant Biotechnol 30:135-141   DOI   ScienceOn
17 Park SY, Ryu SH, Kwon SY, Kim JG, Kwak SS (2004) Molecular cloning of a cytosolic ascorbate from cell cultures of sweetpotato and its expression in response to stress. Mol Genet Genomics (in press)
18 Welinder KG (1992) Superfamily of plant, fungal, and bacterial peroxidases. Curr Opin Struct Biol 2: 388-393   DOI   ScienceOn
19 Welinder KG, Justesen AF, Kjasrsgard IVH, Jensen RB, Rasmussen SK, Jespersen HM, Duroux L (2002) Structural diversity and transcription of class III peroxidases from Arabidopsis thaliana. Eur J Biochem 269:6063-6081   DOI   ScienceOn
20 Xu Y, Chang PLC, Liu D, Narashimhan ML, Kashchandra GR, Hasegawa PM, Bressan RA (1994) Plant defense genes are synergistically induced by ethylene and methyl jasmonate. Plant Cell 6: 1077-1085   DOI   ScienceOn
21 Roberts E, Kutchan T, Kolattukudy PE (1988) Cloning and sequencing of cDNA for a highly anionic peroxidase from potato and the induction of its mRNA in suberizing potato tubers and tomato fruits. Plant Mol Biol 11:15-26   DOI   ScienceOn
22 Schenk PM, Kazan K, Wilson I, Anderson JP, Richmond T, Somerville SC, Manners JM (2000) Coordinated plant defense responses in Arabidopsis revealed by microarray analysis. Proc Natl Acad Sci USA 97:11655-11660   DOI   ScienceOn
23 Tognolli M, Penel C, Greppin H, Simon P (2002) Analysis and expression of the class III peroxidase large gene family in Arabidopsis thaliana. Gene 288:129-138   DOI   ScienceOn
24 Vignutelli A, Wastemack C, Apel K, Bohlmann H (1998) Systemic and local induction of an Arabidopsis thionin gene by wounding and pathogens. Plant J 14: 285-295   DOI   ScienceOn
25 Kim SK, Kwak SS, Jung KH, Min SR, Park IH, Liu JR (1994) Selection of plant cell lines for high yields of peroxidase. J Biochem Mol Biol 27:132-137
26 Ye XS, Pan SQ, Kuc J (1990) Activity, isozyme pattem, and cellular localization of peroxidase as related to systemic resistance of tobacco to blue mold (Peronospora tabacina) and to tobacco mosaic virus. Physiol Biochem 80:1295-1299
27 Zimmerlin A, Wojtaszek P, Bolwell GP (1994) Synthesis of dehydrogenation polymers of ferulic with highly specificity by a purified cell wall peroxidase from French bean (Phaseolus uulgaris L.). Biochem J 299: 747-75
28 Kim KY, Huh GH, Lee HS, Kwon SY, Hur Y, Kwak SS (1999) Molecular characterization of cDNAs for two anionic peroxidases from suspension cultures of sweetpotato. Mol Gen Genet 261: 941-947   DOI   ScienceOn
29 Hiraga S, Sasaki K, Ito H, Ohashi Y, Matsui H (2001) A large family of class III plant peroxidases. Plant Cell Physiol 42: 462-468   DOI   ScienceOn
30 Huh GH, Lee SJ, Bae YS, Liu JR, Kwak SS (1997) Molecular cloning and characterization of cDNAs for anionic and neutral peroxidase from suspension cultured cells of sweetpotato and their differential expression in response to stress. Mol Gen Genet 255: 382-391   DOI   ScienceOn
31 Kim KY, Kwon HK, Kwon SY, Lee HS, Hur Y, Bang JW, Choi KS, Kwak SS (2000) Differential expression of four sweet potato peroxidase genes in response to abscisic acid and ethephon. Phytochemistry 54:19-22   DOI   ScienceOn
32 Kim KY, Kwon SY, Lee HS, Hur Y, Bang JW, Kwak SS (2003) A novel oxidative stress-inducible peroxidase promoter from sweetpotato: molecular cloning and characterization in transgenic tobacco plants and cultured cells. Plant Mol Biol 51:831-838   DOI   ScienceOn
33 Klotz KL, Liu TT, Liu L, Lagrimini LM (1998) Expression of the tobacco anionic peroxidase gene is tissue-specific and developmentally regulated. Plant Mol Biol 36: 509-520   DOI   ScienceOn
34 Kunkel BN, Brooks DM (2002) Cross talk between signalling pathways in pathogen defense. Curr Opin Plant Biol 5:325-331   DOI   ScienceOn
35 Kwak SS, Kim SK, Lee MS, Jung KH, Park IH, Liu JR (1995) Acidic peroxidases from suspension-cultures of sweetpotato. Phytochemistry 39: 981-984   DOI   ScienceOn
36 Omann F, Beauliew N, Tyson H (1993) cDNA sequence and tissue specific expression of an anionic peroxidase. Genome 37: 137-147   DOI   ScienceOn
37 Niki T, Mitsuhara I, Seo S, Ohtsubu N, Ohashi Y (1998) Antagonistic effect of salicylic acid and jasmonic acid on the expression of pathogenesis-related (PR) Protein genes in wounded mature tobacco leaves. Plant Cell Physiol 39: 500-507   DOI   ScienceOn