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http://dx.doi.org/10.5010/JPB.2004.31.1.073

In Vitro Mitotic Chromosome Doubling by Chemical Treatments in Lilium longiflorum  

Chung, Mi-Young (Department of Horticulture, Kyungpook National University)
Chung, Jae-Dong (Department of Horticulture, Kyungpook National University)
J. M. VanTuyl (Business Unit Biodiversity and Breeding, Plant Research International, Wageningen University and Research Center)
Lim, Ki-Byung (Genomics Division, National Institute of Agricultural Biotechnology)
Publication Information
Journal of Plant Biotechnology / v.31, no.1, 2004 , pp. 73-78 More about this Journal
Abstract
This study was carried out to develop an in vitro chromosome doubling systems for the breeding of tetraploid lily cultivar. Different concentration (ppm) and treatment time (hour) of colchicine, oryzalin and caffeine were compared for the efficiency of bulb regeneration and tetraploidization. The occurrence of tetraploid plants by colchicine was the highest in the concentration of 1,000 ppm for three hours. In oryzalin treatment, the best combination was at 30 ppm for three hours. However, the effects of oryzalin treatments were similar between 10 ppm and 30 ppm concentrations. The survival rate was dramatically reduced in a high concentration of caffeine although some treatments had a higher tetraploid induction. As a consequence, reliable results for the tetraploid production were obtained in the treatments of 1,000 ppm colchicine, 3,000 ppm oryzalin both for three hours, and 9,000 ppm caffeine for nine hours.
Keywords
Cholchicine; caffeine; oryzalin; ploidy analysis; tetraploidization;
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