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http://dx.doi.org/10.5010/JPB.2004.31.1.067

Effect of Casein on Somatic Embryogenesis and Plant Regeneration in Shoot Apical Meristem Explants of Sweetpotato (Ipomoea batatas L.)  

Shin, Kong-Sik (National Institute of Agricultural Biotechnology, RDA)
Roh, Kyung-Hee (National Institute of Agricultural Biotechnology, RDA)
Lee, Yeon-Hee (National Institute of Agricultural Biotechnology, RDA)
Park, Young-Whan (National Institute of Agricultural Biotechnology, RDA)
Suh, Seok-Cheol (National Institute of Agricultural Biotechnology, RDA)
Publication Information
Journal of Plant Biotechnology / v.31, no.1, 2004 , pp. 67-72 More about this Journal
Abstract
An efficient protocol has been developed for rapid mass propagation of sweetpotato from shoot-tips derived embryogenic callus. Optimal embryogenic callus was induced from shoot apical meristem explants on Murashige and Skoog (MS) medium supplemented with 1mg/L 2,4-D. The addition of casein hydrolysate in the media increased the embryogenesis efficiency of sweetpotato. Somatic embryos were easily induced from the embryogenic callus on MS basal medium containing 300-500mg/L casein hydrolysate without phytohormon. Treatment of casein hydrolysate (100∼300mg/L) with 1mg/L 2,4-D also improved the secondary embryonic efficiency from somatic embryos below 2mm in length. Plant regeneration was achieved via somatic embryogenesis and direct organogenesis. Regenerated planlets with well developed shoots and roots on MS basal medium were successfully transferred to soil.
Keywords
Casein hydrolysate; embryogenic callus; shoot apical meristem; somatic embryo; somatic embryogenesis; sweetpotato;
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