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http://dx.doi.org/10.5010/JPB.2004.31.1.025

Effects of Growth Regulators on Callus Induction and Plant Regeneration from Leaf Explants of Sedum sarmentosum  

Ahn, Jeong-Ho (Institute of Life Science and Natural Resources, Division of Plant Resources Science)
Lee, Seung-Yeob (Institute of Life Science and Natural Resources, Division of Plant Resources Science)
Publication Information
Journal of Plant Biotechnology / v.31, no.1, 2004 , pp. 25-29 More about this Journal
Abstract
To establish the system of in uitro plant regeneration, the leaf segments of Sedum sarmentosum were cultured on MS media supplemented with different levels of 2,4-D, NAA and BA. The callus induction and growth showed a good response on MS medium supplemented with 3.0mg/L 2,4-D and 1.0mg/L BA, but a few callus induced on medium containing NAA and BA. In plant regeneration, combination of BA and NAA promoted shoot organogenesis from callus, and the highest frequency was obtained on MS medium supplemented with 0.2mg/L NAA and 3.0mg/L BA. When calli were transferred to the plant regeneration medium containing 0.2mg/L NAA and 3.0mg/L BA, healthy shoots without hyperhydricity were continuously induced (17.2 plantlets per callus) after 50 days of culture. When regenerated plantlets were transferred onto hormone-free MS medium, rooting was easily achieved from all of them.
Keywords
Callus; growth regulator; plant regeneration; Sedum sarmentosum;
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Times Cited By KSCI : 3  (Citation Analysis)
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1 Choi HK, Son JS, Na QH, Hong SS, Park YS, Song JY (2002) Mass production of paclitaxel by plant cell culture. Kor J Plant Biotech 29:59-62   DOI   ScienceOn
2 Kim OT, Kim KS, Ahn JC, Hwang B (2001) Plant regeneration uia somatic embryogenesis and organogenesis from Peucedanum japonicum Thunb. Kor J Plant Biotech 28: 21-24
3 Kim OT, Kim MY, Park YJ, Hong MH, Ahn JC, Oh MH, Hwang B (2002) Production of triterpene glycosides from whole plant cultures of Centella asiatica (L.) Urban. Kor J Plant Biotech 29: 282-285
4 Koroch A, Juliani HR, Kapteyn J, Simon JE (2002) In uitro regeneration of Echinacea purpurea from leaf explants. Plant Cell Tiss Org Cult 69: 79-83   DOI   ScienceOn
5 Kwon ST, Jeong JH (1999) Genetic relationship among Sedum species based on morphological characteristics and RAPD analysis. Kor J Hort Sci Tech 17:490-494
6 Skoog F, Strong FM, Miller CO (1965) Cytokinins. Science 148: 532 533
7 Choi SCI, Nam SH, Yang OJ, Cho MJ, Yang MS (1994) Plant regeneration from the stem tissue of Orostachys japonicus A. Berger. Kor J Plant Biotech 21:65-68
8 Devlin RM (1975) Plant growth hormones, In: Plant physiology (3rd), (ed) D. Van Nortrand Company, New York, pp. 411 -517
9 Lee SY, Yoo SO, Bae JH, Lee JH (2002) Effect of plant growth regulators on callus induction and plant regeration of Farfugium japonica. Kor J Plant Biotech 29:45-49   DOI   ScienceOn
10 Lisowska K, Wysokinska H (2000) In. uitro propagation of Catalpa ovata G. Don. Plant Cell Tiss Org Cult 60:171 -176   DOI   ScienceOn
11 Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:473-497   DOI
12 Rha ES, Kim JL (1996) Plant regeneration in leaf explant cultuers of Reltmannia. glutinosa Liboschuitz. Kor J Plant Biotech 23: 299- 302
13 Ryu JH, Doo HS, Kwon TH (1992) Induction of haploid plants by anther culture in sesame (Sesam.um indicum L.) I. Effects of growth regulators and difference between genotypes on callus induction. Kor J Plant Biotech 19:171-177
14 Yoon ES (1996) Effect of polyvinylpyrrolidone on callus growth and plant regeneation of Pulsatilla Koreana. Kor J Plant Tiss Cult 23: 349-354
15 Yoon ES (1997) Effect of plant growth regulators on plant regeneration from leaf and stem explant culture of Sedum erythrostichum Miq. Kor J Plant Biotech 24: 285-289
16 Seo MS, Bae CH, Choi DO, Rhim SL, Seo SC, Song PS, Lee HY (2002) Investigation of transformation efficiency of rice using Agrobacterium tumefaciens and high transformation of GPAT (glycerol-3-phosphate acyltransferase) gene relative to chilling tolerance. Kor J Plant Biotech 29:85-92   DOI   ScienceOn