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http://dx.doi.org/10.5010/JPB.2004.31.1.025

Effects of Growth Regulators on Callus Induction and Plant Regeneration from Leaf Explants of Sedum sarmentosum  

Ahn, Jeong-Ho (Institute of Life Science and Natural Resources, Division of Plant Resources Science)
Lee, Seung-Yeob (Institute of Life Science and Natural Resources, Division of Plant Resources Science)
Publication Information
Journal of Plant Biotechnology / v.31, no.1, 2004 , pp. 25-29 More about this Journal
Abstract
To establish the system of in uitro plant regeneration, the leaf segments of Sedum sarmentosum were cultured on MS media supplemented with different levels of 2,4-D, NAA and BA. The callus induction and growth showed a good response on MS medium supplemented with 3.0mg/L 2,4-D and 1.0mg/L BA, but a few callus induced on medium containing NAA and BA. In plant regeneration, combination of BA and NAA promoted shoot organogenesis from callus, and the highest frequency was obtained on MS medium supplemented with 0.2mg/L NAA and 3.0mg/L BA. When calli were transferred to the plant regeneration medium containing 0.2mg/L NAA and 3.0mg/L BA, healthy shoots without hyperhydricity were continuously induced (17.2 plantlets per callus) after 50 days of culture. When regenerated plantlets were transferred onto hormone-free MS medium, rooting was easily achieved from all of them.
Keywords
Callus; growth regulator; plant regeneration; Sedum sarmentosum;
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Times Cited By KSCI : 3  (Citation Analysis)
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