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http://dx.doi.org/10.5010/JPB.2003.30.4.315

Transformation of Artemisia adamsii, Endemic to a Gobi Desert, with CLP, Dhn5 to Enhance Environmental Stress Tolerance  

Han, Kyu-Hyun (School of Bioresource Sciences, Dankook University)
Hwang, Cheol-Ho (School of Bioresource Sciences, Dankook University)
Publication Information
Journal of Plant Biotechnology / v.30, no.4, 2003 , pp. 315-321 More about this Journal
Abstract
Freezing and drought tolerances in plants are very important for survival in the desert. In an effort to reduce desertifcation in Gobi, a molecular breeding of Artemisia adamsii using the CLP (chitinase like protein, antifreeze protein) and Dhn5 (dehydrin5) genes from barley is performed by introducing them into Artemisia adamsii via Agrobacteria. We had found an optimal combinatorial concentration of hormones at 0.05mg/L of NAA and 0.5mg/L of BA for growth of callus in Artemisia adamsii. In addition, the higher rate of callus induction using hypocotyl as explant was observed comparing to explants of stem and leaf. There were some variations in the level of the proteins expressed among the transgenic lines such that the lines of CLP(CS1-5, 1-7,4-4) and Dhn5(DS2-2, 2-3) lines produce the protein to higher levels. The transgenic lines showing a higher level of Dhn5 exhibited better growth than nontransgenic callus in presence of 10 and 20％ PEG. In case of the CLP tansgenic lines, both CS1-5 and CS1-7 showed a higher level of freezing tolerance determined by ion leakage test.
Keywords
Artemisia adamsii; CLP; dehydrin5; drought tolerance; freezing tolerance; Gobi desert;
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