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http://dx.doi.org/10.5010/JPB.2003.30.3.301

Effect of Nitrogen Source on the Cell Growth and Production of Secondary Metabolites in Bioreactor Cultures of Eleutherococcus senticosus  

Ahn, Jin-Kwon (Plant Biotechnology Division, Forest Genetic Resources Department, Korea Forest Research Institute)
Lee, Wi-Young (Plant Biotechnology Division, Forest Genetic Resources Department, Korea Forest Research Institute)
Park, So-Young (Plant Biotechnology Division, Forest Genetic Resources Department, Korea Forest Research Institute)
Publication Information
Journal of Plant Biotechnology / v.30, no.3, 2003 , pp. 301-305 More about this Journal
Abstract
The effects of inorganic nitrogen sources such as KNO$_3$ and NH$_4$ NO$_3$ on cell growth and production of chlorogenic acid and eleutheroside E derivative were investigated in 5L bioreactor cultures of Eleutherococcus senticosus. The cell growth in the 1/2MS medium containing 15mMKNO$_{3}$. The fresh weight of cells harvested from bioreactor was affected by the concentration ratio of NO$_3$$^{[-10]}$ and NH$_4$$^{+}$ in culture medium. At the viewpoint of secondary metabolite production, the production of chlorogenic acid was affected by the concentration of NH$_4$$^{+}$ in the culture medium, but not by the total concentration of nitrogen sources in the culture medium. Futhermore, eleutheroside E derivative production was also affected by the concentration ratio of NO$_3$$^{[-10]}$ and NH$_4$$^{+}$ in the culture medium. Base on those results, it is suggested that cell growth and production of secondary metabolite(chlorogenic acid and eleutheroside E derivative) could be manipulated by controlling the total concentration of nitrogen sources and the concentration ratio of NO$_3$$^{[-10]}$ and NH$_4$$^{+}$ in the culture medium. medium.
Keywords
Chlorogenic acid; eleutheroside E; NO$_3$$^{[-10]}$; NH$_4$$^{+}$; siberian ginseng;
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Times Cited By KSCI : 1  (Citation Analysis)
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