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http://dx.doi.org/10.5010/JPB.2003.30.3.215

The Effect of agrobacterium Density on Transformation Efficiency in Apple  

Seong, Eun-Soo (National Horticultural Research Institute, RDA)
Cha, Ji-Eun (National Horticultural Research Institute, RDA)
Kim, Jeong-Hee (National Horticultural Research Institute, RDA)
Park, Seong-Whan (Faculty of National Resources and Life Science, Dong-A University)
Yu, Chang-Yeon (College of Agriculture and Life Sciences, Kangwon National University)
Song, Kwan-Jeong (Faculty of Horticultural Life Sciences, Cheju National University)
Publication Information
Journal of Plant Biotechnology / v.30, no.3, 2003 , pp. 215-219 More about this Journal
Abstract
This study was conducted to find optimum bacterial density for improving the efficiency of transformation mediated by Agrobacterium tumefaciens in apples. Regeneration(15％) and transformation frequency(10％) were increased in resuspension-culture density $A_{600}$ 1.3 from preculture density $A_{600}$ 0.7 of Agrobacterium tumefaciens in ′Fuji′. In ′Gala′, 20% regeneration and 16% transformation frequency were observed at optimum bacterial density $A_{600}$ 0.7 form preculture density $A_{600}$ 1.3. ′Mclntosh as well as "Gala" were 25％regeneration and 10％ transformation frequency. Hence a frequency optimum condition of bacterial density for the efficient transformation of apple could be depend on apple genotypes.
Keywords
Bacterial density; absorbance; regeneration frequency; transformation frequency;
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Times Cited By KSCI : 1  (Citation Analysis)
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1 Archilletti T, Lauri P, Damian C (1995) Agrobacterium-mediated transformation of almond leaf pieces. Plant Cell Rep 14: 267-272
2 Bolar JP, Brown SK, Norelli JL, Aldwinckle HS (1999) Factors affecting the transformation of 'Marshall Mclntosh' apple by Agrobacterium tumefaciens. Plant Cell Tiss Org Cult 55: 31-38   DOI   ScienceOn
3 Mondal TK, Bhattacharya A, Ahuja PS, Chard PK (2001) Transgenic tea [Camellia sinensis (L.) O. kuntze cv. Kangra Jat] plants obtained by Agrobacterium-mediated transformation of somatic embryos. Plant Cell Rep 20: 712-720   DOI   ScienceOn
4 Pena L, Cervera M, Juarez J, Ortega C, Pina JA, Duran-Vila N, Navarro L (1995) High efficiency Agrobacterium-mediated transformation and regeneration of Citrus. Plant Sci 104: 183-191   DOI   ScienceOn
5 Puite L, Schaart JG (1996) Genetic modification of the commercial apple cultivars Gala, Golden Delicious and Elstar via an Agrobacterium-mediated transformation method. Plant Sci 119: 125-133   DOI   ScienceOn
6 Humara JM, LopezM, Ordas RJ (1999) Agrobacterium tumefaciens mediated transformation of Pinus pinea L cotyledons: an assessment of factors influencing the efficiency of uidA gene transfer. Plant Cell Rep 19: 51-58   DOI   ScienceOn
7 James DJ, Dandekar AM (1991) Regeneration and transformation of apple (Malus pumila Mill.). Plant Tissue Culture Manual B8 (1-18). Kluwer Academic Publishers, the Netherlands
8 James DJ, Passey AJ, Rugini E (1988) Factors affecting high frequency plant regeneration from apple tissues cultured in vitro. J Plant Physiol 132: 149-154
9 James DJ, Uratsu S, Cheng J, Negri P, Viss P, Dandekar AM (1993)Acetosyringone and osmoprotectants like betaine or proline synergistically enhance Agrobacterium-mediated transformation of apple. Plant Cell Rep 12: 559-563
10 Kumria R, Waiw B, Rajam MV (2001) Plant regeneration from transformed embryoqenic callus of an elite indica rice via Agrobacterium. Plant Cell Tiss Org Cult 67: 63-71   DOI   ScienceOn
11 Mireille C, Joan EP, Frank C, Vicky BW (1988) Parameters affecting the frequency of kanamycin resistant alfalfa obtained by Agrobacterium-mediated transformation. Plant Cell Rep 7: 512-516   DOI   ScienceOn
12 Welander M (1988) Plant regeneration from leaf and stem segments of shoots raised in vitro from mature apple trees. J Plant Physiol 132: 738-744   DOI
13 De Bondt A, Eggermont K, Druart P, De Vil M, Goderis I, Vanderleyden J, Broekaert WF (1994) Agrobacterium-mediated transformation of apple (Malus $\times$ domestica Borkh.): an assessment of factors affecting gene transfer efficiency during early transformation steps. Plant Cell Rep 13: 587-593   DOI   ScienceOn
14 Dufour M (1990) lmproving yield of adventitious shoot in apple. Acta Horticulturae 280: 51-60
15 Howe GT, Goldfarb B, Stranss F (1994) Agrobacterium-mediated transformation of hybrid poplar suspension cultures and regeneration of transformed plants. Plant Cell Tiss Org Cult 36: 59-71   DOI   ScienceOn