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http://dx.doi.org/10.5010/JPB.2003.30.2.123

Cytogenetic Analyses of Angelica Plants Using Feulgen Staining and Multicolor Fluorescence in Situ Hybridization  

Koo, Dal-Hoe (Department of Biology, Chungnam National University)
Kim, Soo-Young (Department of Biology, Chungnam National University)
Bang, Kyong-Hwan (Industrial Crop Division, National Crop Experimental Station, RDA)
Seong, Nak-Sul (Industrial Crop Division, National Crop Experimental Station, RDA)
Bang, Jae-Wook (Department of Biology, Chungnam National University)
Publication Information
Journal of Plant Biotechnology / v.30, no.2, 2003 , pp. 123-127 More about this Journal
Abstract
Karyotype analysis and chromosomal localization of 5S and 45S rDNAs using multi-color fluorescence in situ hybridization (McFISH) technique were carried out in two Angelica species. The numbers of diploid chromosomes were the same in two same in two species as 2n=22, however the lengths of chromosomes were varied from 4.25 to 6.50 ${\mu}{\textrm}{m}$ in A gigas and 4.95 to 8.50 ${\mu}{\textrm}{m}$ in A acutiloba. The chromosomes of A. gigas were composed of five metacentric and six submetacentric pairs, while those of A. acutiloba were six metacentic, one submetacentric and four subtelocentric paris. In FISH experiments, the numbers and size of 45S rDNA signals were varied between two species, however dach signal of the 5S rDNA was observed in two species.
Keywords
Angelica species; karyotype; multi-color fluorescence in situ hybridization;
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