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http://dx.doi.org/10.5010/JPB.2003.30.1.041

Effects of Light, Temperature, and Sucrose on Plant Regeneration from the Flower Organ Explant in Iris ensata  

Yoon, In-Kyung (Department of Life Resources, Catholic University of Daegu)
Koh, Jae-Chul (Department of Life Resources, Catholic University of Daegu)
Publication Information
Journal of Plant Biotechnology / v.30, no.1, 2003 , pp. 41-45 More about this Journal
Abstract
A study was under taken to investigate the appropriate explant sources of flower organ and suitable cultural conditions such as light, temperature, and sucrose in plant regeneration of Iris ensata culture. Explants of perianth, ovary, pedicel, and peduncle of Iris ensata were cultured at different daylength (0, 8, 16, 24 hour), different temperatures (10, 15, 25, 3$0^{\circ}C$), and sucrose concentrations (1, 3, 6, 9%) on MS medium. Formation of adventitious roots from explants of Iris ensata was effective in the dark, while that of adventitous shoots was effective in the light. The optimum daylength for young plant regeneration was 16 hours. The optimum temperature for shoot formation of Iris ensata explants was $25^{\circ}C$ but the formation at 10 and 15$^{\circ}C$ was ineffective. Especi-ally, perianth and ovary was effective in shoot formation from flower organ expants. T-he optimum concentration of sucrose for shoots and roots formation of Iris ensata explants was 3 and 6%, respectively.
Keywords
Floral explant; in vitro; light; mutiple shoots; sucrose; temperature;
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