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http://dx.doi.org/10.5010/JPB.2003.30.1.027

Effects of Hormone and Several Factors on the Regeneration and Transformation rate of Potato Cultivars Bred in Korea  

Yi, Jung-Yoon (Highland Crop Research Division, National Highland Agricultural Experiment Station, RDA)
Seo, Hyo-Won (Highland Crop Research Division, National Highland Agricultural Experiment Station, RDA)
Cho, Ji-Hong (Highland Crop Research Division, National Highland Agricultural Experiment Station, RDA)
Lee, Shin-Woo (Department fo Crops Biotechnology, Jinju National University)
Yun, Han-Dae (Division of Applied Life Science, Gyeongsang National University)
Publication Information
Journal of Plant Biotechnology / v.30, no.1, 2003 , pp. 27-33 More about this Journal
Abstract
The optimal condition of in vitro regeneration and transformation were investigated for newly bred potato varieties in Korea. Leaf and internodal stem tissues of 'Chubak', 'Namsuh', 'Jasim', 'Jopung' and 'Jowon' were used to investigate the influence of growth regulator on regeneration efficiency The effect of phenolic compound acetosyringone on gene transformation efficiency was also investigated. Leaf tissue of 'Jowon' and internodal stem tissues of 'Jopung' were showed high regeneration efficiency on M5 medium supplemented with 0.1 mg/L GA₃, 2.0 mg/L Zeatin and 0.01 mg/L NAA. The other three potato cultivars were showed low regeneration efficiency less than 25%. The effect of acetosyringone on Agrobacterium-mediated gene transformation with leaf and internodal stem tissues were noticeable. By adding the 75 μM of acetosyringone during the Agrobacterium innoculation, the transformation efficiency was increased up to 1.5∼4.0 fold compare to non-treated control. In case of 'Jowon' the transformation efficiency was 87.9% in leaf tissue and 'Jopung' was 68.4% in internodal stem tissues.
Keywords
Acetosyringone; growth regulator; potato; transformation;
Citations & Related Records
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