Browse > Article
http://dx.doi.org/10.5010/JPB.2002.29.4.223

Efficient Gene Introduction into Rice Callus by Using Particle Inflow Gun System  

Song, In-Ja (College of Agricultural & Life Science, Sunchon National University)
Bae, Chang-Hyu (College of Agricultural & Life Science, Sunchon National University)
Choi, Dae-Ock (College of National Science, Sunchon National University)
Ryo Akashi (Division of Grassland and Animal Production, Faculty of Agriculture, Miyazaki University)
Lee, Hyo-Yeon (College of Agricultural & Life Science, Sunchon National University)
Publication Information
Journal of Plant Biotechnology / v.29, no.4, 2002 , pp. 223-228 More about this Journal
Abstract
We have developed a homemade particle inflow gun (PIG) system which has simple operation method, low price and high gene introduction efficiency into rice callus. Rice callus were inflowed with gold particles containing DNA of a plasmid, pIG121Hm, harboring intron GUS ($\beta$-glucuronidase) gene, NPTII gene and HPT gene. For optimal GUS transient expression, the effects of parameters on DNA delivery efficiency of the PIG system was investigated by scoring transient GUS expression. The highest number of blue spots was observed at 16 mM of spemidine and 1.5 M of calcium chloride, respectively. And the amount of gold particles required for the best GUD expression was 2 mg. Optimum GUS transient expression was observed at target distance of 12 cm and helium pressure of 3.5 bar (50 psi). Gene introduction efficiency of the PIG system was observed almost similar to that of the Biolistic Gun (Bio-Rad Company). Since PIG system is simple to operate and one doesn't need disposable accessaries, the PIG system can be easily applied to various replication experiments.
Keywords
Calcium chloride; gold particles; helium pressure; shoting distance; spermidine;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Brewer EP, Saunders JA, Angle JS, Chaney RL, Mclntosh MS (1999) Somatic hybridization between the zinc accumulator Thtaspi caerulescens and Brassica napus. Theor Appl Genet 99:761-771   DOI   ScienceOn
2 Chu CC, Wang CC, Sun CS, Hsu C, Yin KC, Chu CY, Bi FY (1975) Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen sources. Sci Sin 18:659-668
3 Dhir SK, Oglesby J, Bhagsari AS (1998) Plant regeneration via somatic embryogenesis, and transient gene expression in sweet potato protoplasts. Plant Cell Rep 17:665-669   DOI   ScienceOn
4 Fmer JJ, Vain P, Jones MW, McMullen MD (1992) Development of particle inflow gun for DNA delivery to plant cells. Plant Cell Rep 11:323-328
5 Hiei Y, Ohta S, Komari T, Kumashiro T (1994) Efficient transformation of rice (Oryza satiua L.) mediated by agrobacterium and sequence analysis of the boundaries of the T-DNA. Plant J 6:271 -282   DOI   ScienceOn
6 Jefferson RA (1987) Assaying chimeric genes in plant :the GUS gene fusion system. Plant Mol Biol Rep 5:397-405
7 Kexuan T, Porntip T, Yanan X, Xiaofen S, John AG, Pamela CR, Huaxiong Q, Xinggui L, Paul C, Ajay K (1999) Particle-bombardment-mediated co transformation of elite Chinese rice cultivars with genes conferring resistance to bacterial blight and sap-sucking insect pests. Planta 208:552-563   DOI   ScienceOn
8 Lee HY, Lee CH, Kim HI, Han WD, Choi JE, Kim JH, Lim YP (1998) Development of bialaphos-resistant transgenic rice using Agrobacterium tumefaciens. Korean J Plant Tiss Cult 25:283-288
9 Radloff R, Bauer W, Vinograd J (1967) A dye-buoyant-density method for the detection and isolation of closed circular duplex DNA-the original description of ethidium bromide density gradient centrifugation. Proc Natl Acad Sci USA 57:1514-1521   DOI   ScienceOn
10 Rasco Gaunt S, Riley A, Barcelo P, Lazzeri PA (1999) Analysis of particle bombardment parameters to optimize DNA delivery into wheat tissues. Plant Cell Rep 19:118-127   DOI   ScienceOn
11 Seo MS, Bae CH, Choi DO, Rhim SL, Seo SC, Song PS, Lee HY (2002) Investigation of transformation efficiency of rice using Agrobacterium tumefaciens and high transformation of GPAT (glycerol-3-phosphate acyltransferase) gene relative to chilling tolerance. Korean J Plant Tiss Cult 29:85-92
12 Rashid H, Yokoi S, Toryama K, Hinata K (1996) Transgenic plant production mediated by Agrobacterium tumefaciens in Indica rice. Plant Cell Rep 15:727-730   DOI   ScienceOn
13 Russell JA, Roy MK, Sanford JC (1992) Physical trauma and tungsten toxicity reduce the efficiency of biolistic transformation. Plant Physiol 98:1050-1056   DOI   ScienceOn
14 Sanford JC (1990) Biolistic plant transformation. Physologia Plantarum 79:206-209   DOI   ScienceOn
15 Snyder GW, Ingersoll JC, Smigocki AC, Owens LD (1999) Introduction of pathogen defense genes and a cytokinin biosynthesis gene into sugarbeet (Beta uulgaris L.) by Agrobacterium or particle bombardment. Plant Cell Rep 18:829-834   DOI   ScienceOn
16 Vera H, Ulrich K (1998) Splicing of the mitochondrial group-II intron rl1:conserved intron-exon interacti ons diminish splicing effciency.Curr Genet 33:117-123   DOI   ScienceOn
17 Taylor MG, Vasil IK (1991) Histology of and physical factors affecting transient GUS expression in pearl millet (Pennisetum glacum (L.) R. Br.) embryos following microprojectile bombardment. Plant Cell Rep 10:120-125
18 Klein TM, Gradziel T, Fromm ME, Sanford JC (1988) Factors influencing gene delivery into Zea mays cells by high-velocity microprqjectiles. Bio/Technol 6:559-563   DOI