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http://dx.doi.org/10.5010/JPB.2002.29.2.111

Plant Regeneration and Effect of Auxin and Cytokinin on Adventitious Shoot Formation from Seedling Explant of Taraxacum platycarpum  

Lee, Mi-Hyun (Department of Biological Science, Kong-Ju National University)
Yoon, Eui-Soo (Department of Biological Science, Kong-Ju National University)
Jung, Su-Jin (Department of Biological Science, Kong-Ju National University)
Bae, Ki-Hwa (Department of Biological Science, Kong-Ju National University)
Seo, Jin-Wook (Department of Biotechnology, Chung-Ang University)
Choi, Youg-Eui (Korea Ginseng Institute, Chung-Ang University)
Publication Information
Journal of Plant Biotechnology / v.29, no.2, 2002 , pp. 111-115 More about this Journal
Abstract
Taraxacum platycarpum has been used as a medicinal plant. We investigated optimal condition for efficient plant regeneration through adventitious shoot formation on medium with various kinds of growth regulators. Adventitious shoot formation was achieved when cytokinin was used alone. Shoot formation was higher on MS medium containing 2 mg/L BAP compared to that with 2 mg/L kinetin and 2 mg/L 2-ip. Among root, hypocotyl and cotyledon, roots were the best explant for the adventitious shoot induction. Adventitious shoot formation from roots declined markedly by the combination of both 0.1 mg/L NAA and 2 mg/L BAP, while shoot formation from cotyledons was stimulated by the same combination. Root formation from the regenerated shoots was achieved on 1/3MS medium containing 0.2 mg/L NAA. Regenerated plantlets was acclimatized and transplanted to the soil, showing 100% survival.
Keywords
Taraxacum platycarpum; plant regeneration; shoot formation;
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