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http://dx.doi.org/10.12791/KSBEC.2022.31.3.170

Changes in Abscisic Acid, Carbohydrate, and Glucosinolate Metabolites in Kimchi Cabbage Treated with Glutamic Acid Foliar Application under Extremely Low Temperature Conditions  

Sim, Ha Seon (Department of Horticultural Science, College of Agricultural and Science, Kyungpook National University)
Jo, Jung Su (Institute of Agricultural Science and Technology, Kyungpook National University)
Woo, Ui Jeong (Department of Horticultural Science, College of Agricultural and Science, Kyungpook National University)
Moon, Yu Hyun (Department of Horticultural Science, College of Agricultural and Science, Kyungpook National University)
Lee, Tae Yeon (Department of Horticultural Science, College of Agricultural and Science, Kyungpook National University)
Lee, Hee Ju (Vegetable Research Division, National Institute of Horticultural and Herbal Science)
Wi, Seung Hwan (Vegetable Research Division, National Institute of Horticultural and Herbal Science)
Kim, Sung Kyeom (Department of Horticultural Science, College of Agricultural and Science, Kyungpook National University)
Publication Information
Journal of Bio-Environment Control / v.31, no.3, 2022 , pp. 170-179 More about this Journal
Abstract
Glutamic acid is a precursor of essential amino acids that play an important role in plant growth and development. It is one of the biostimulants that reduce cold stress damage by stimulating biosynthetic pathways leading to cryoprotectants. This study evaluated the effects of glutamic acid foliar application on Kimchi cabbage under low-temperature stress. There were six treatments, combining three photo-/dark periods temperature levels (11/-1℃ extremely low, E; 16/4℃ moderately low, M; and 21/9℃ optimal, O) with and without glutamic acid foliar application (0 and 10 mg·L-1; Glu 0 and Glu 10). Glutamic acid foliar application was sprayed once 10 days after transplanting, and then temperature treatment immediately after glutamic acid foliar application was conducted for up to four days. After four days of treatment, abscisic acid (ABA), phaseic acid (PA), dihydrophaseic acid (DPA), and abscisic acid-glucose ester (ABA-GE) contents were higher with Glu 10 treatment than Glu 0 treatment in M treatment. Glucose content was highest in E with Glu 10 treatment (52.1 mg·100 g-1 dry weight), while fructose content was highest in O with Glu 0 treatment (134.6 mg·100 g-1 dry weight). The contents of glucolepiddin (GLP), glucobrassicin (GBS), 4-methoxyglucobrassicin (4MGBS), neoglucobrassicin (GNBS), and gluconasturtiin (GNS) were highest among all treatments in E with Glu 10 treatments (0.72, 2.05, 1.67, 9.40 and 0.85 µmol·g-1 dry weight). After two days of treatment, rapid changes in PA and DPA contents of E with Glu 10 treatments were confirmed, and several individual glucosinolate contents (GLP, GBS, 4MGBS, GNBS, and GNS) were significantly different depending on low temperature and glutamic acid treatment. In addition, the content of fructose was significantly lower than that of O treatment in E and M treatments after four days of treatment. Therefore, although the changes in PA, DPA, glucose, fructose, and individual glucosinolates according to low temperature and glutamic acid foliar treatment were shown. A clear correlation between low temperature and glutamic acid effects could not be evaluated. Results indicated that Brassica crops are cryophilic vegetables, do not react sensitively to low temperatures, and mostly have cold resistance.
Keywords
ABA metabolite; biostimulant; Brassica rapa L. subsp. pekinensis (Lour.) Hanelt; low temperature stress; phytochemical;
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