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http://dx.doi.org/10.12791/KSBEC.2021.30.1.085

Changes in Growth and Bioactive Compounds of Lettuce According to CO2 Tablet Treatment in the Nutrient Solution of Hydroponic System  

Bok, Gwonjeong (Department of Horticulture Science, College of Agriculture & Life Sciences, Chungnam National University)
Noh, Seungwon (Department of Horticulture Science, College of Agriculture & Life Sciences, Chungnam National University)
Kim, Youngkuk (Department of Computer Science and Engineering, College of Engineering, Chungnam National University)
Nam, Changsu (Department of Horticulture Science, College of Agriculture & Life Sciences, Chungnam National University)
Jin, Chaelin (Department of Horticulture Science, College of Agriculture & Life Sciences, Chungnam National University)
Park, Jongseok (Department of Horticulture Science, College of Agriculture & Life Sciences, Chungnam National University)
Publication Information
Journal of Bio-Environment Control / v.30, no.1, 2021 , pp. 85-93 More about this Journal
Abstract
In hydroponic cultivation, in order to investigate the change of lettuce growth and physiologically active substances through CO2 tablet treatment in nutrient solution, we used a solid carbonated tablets commercially available in the Netherlands. The experiment consisted of 0.5-fold, 1-fold, and 2-fold treatment groups with no treatment as a control. As a result, the atmospheric CO2 concentration in the chamber after CO2 tablet treatment showed the highest value at 472.2 µL·L-1 in the 2-fold treatment zone immediately after treatment, and the pH in the nutrient solution decreased the most to pH 6.03 in the 2-fold treatment zone. After that, over time, the CO2 concentration and pH recovered to the level before treatment. Leaf width and leaf area of lettuce showed the highest values of 17.1cm and 1067.14 ㎠ when treated 2-fold with CO2 tablet, while fresh weight and dry weight of the above-ground part were highest at 63.87 g and 3.08 g in 0.5-fold treatment. The root length of lettuce was the longest (28.4 cm) in the control, but there was no significant difference in the fresh weight and the dry weight among the treatments. Apparently, it was observed that the root length of the lettuce was shortened by CO2 tablet treatment and a lot of side roots occurred. In addition, there was a growth disorder in which the roots turned black, but it was found that there was no negative effect on the growth of the above-ground part. As a result of analyzing the bioactive compounds of lettuce by CO2 tablet treatment, chlorogenic acid and quercetin were detected. As a result of quantitative analysis, chlorogenic acid increased by 249% compared to the control in 1-fold treatment, but quercetin decreased by 37%. As a result of comparing the DPPH radical scavenging ability showing antioxidant activity, the control and 0.5-fold treatment showed significantly higher values than the 1-fold and 2-fold treatments. This suggests that carbonated water treatment is effective in increasing the growth and bioactive compounds of hydroponic lettuce.
Keywords
antioxidant activity; chlorogenic acid; DFT; DPPH; HPLC; quercetin;
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Times Cited By KSCI : 2  (Citation Analysis)
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