Browse > Article
http://dx.doi.org/10.12791/KSBEC.2018.27.3.231

Chlorophyll Fluorescence, Chlorophyll Content, Graft-taking, and Growth of Grafted Cucumber Seedlings Affected by Photosynthetic Photon Flux of LED Lamps  

Kim, Hyeong Gon (Institute for Agricultural Machinery & ICT Convergence, Chonbuk National University)
Lee, Jae Su (Farming Automation Division, Dept. of Agricultural Engineering, National Institute of Agricultural Sciences, RDA)
Kim, Yong Hyeon (Institute for Agricultural Machinery & ICT Convergence, Chonbuk National University)
Publication Information
Journal of Bio-Environment Control / v.27, no.3, 2018 , pp. 231-238 More about this Journal
Abstract
Chlorophyll fluorescence, chlorophyll content, graft-taking and growth of grafted cucumber seedlings as affected by photosynthetic photon flux (PPF) of LED lamps were analyzed in this study. Four PPF levels, namely 25, 50, 100, $150{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ were provided to investigate the effect of light intensity on the chlorophyll fluorescence, chlorophyll content, graft-taking and growth of grafted cucumber seedlings. Air temperature, relative humidity, and photoperiod for graft-taking were maintained at $25^{\circ}C$, 90%, $16h{\cdot}d^{-1}$, respectively. Maximum quantum yield (Fv/Fm) of rootstock as affected by PPF was found to be 0.84-0.85 and there was no significant change in Fv/Fm. Even though Fv/Fm of scion measured at 2 days after grafting was lowered to 0.81-0.82, after then it gradually increased with increasing PPF. At 4 days after grafting, the chlorophyll content extracted from scion increased with increasing PPF. Graft-taking ratio of grafted cucumber seedlings was 90-95% as PPF was ranged from $25{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ to $100{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$. However, the graft-taking ratio of grafted seedlings healed under PPF of $150{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ was decreased to 80%. Maximum PPF measured required for smooth joining of rootstock and scion was assumed to be $100{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$. At healing stage of grafted cucumber seedlings, Fv/Fm of scion decreased and at least two days after grafting were required for rooting of grafted seedlings. Chlorophyll fluorescence response of rootstock and scion was linked to light irradiation. Therefore, it was concluded that physical environment including light and humidity during healing process of grafted seedlings should be controlled more precisely to facilitate root formation and to prevent scion from lowering Fv/Fm. Further studies are required to investigate the effects of root development and joining of vascular bundles of grafted seedlings on the chlorophyll content of scion.
Keywords
chlorophyll; cucumber seedlings; grafting; maximum quantum yield; photosynthetic photon flux;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Hiscox, J.D. and G.F. Israelstam. 1979. A method for the extraction of chlorophyll from leaf tissue without maceration. Can. J. Bot. 571332-1334. doi:10.1139/b79-163   DOI
2 Jang, Y., E. Goto, Y. Ishigami, B. Mun, and C. Chun. 2011. Effects of light intensity and relative humidity on photosynthetic rate, growth and graft-take of grafted cucumber seedlings during healing and acclimatization. Hortic. Environ. Biotechnol. 52:331-338. doi:10.1007/s13580-011-0009-8   DOI
3 Johnson, G.N., A.J. Young, J.D. Scholes, and P. Horton. 1993. The dissipation of excess excitation energy in British plant species. Plant, Cell & Environ. 16:673-679. doi:10.1111/j.1365-3040.1993.tb00485.x   DOI
4 Kim, S.K., H.J. Kim, K.J. Choi, J.H. Lee, G.C. Chung, and S.J. Chung. 2001. Effect of light intensity, temperature and leaf chlorophyll content on photosynthesis and respiration of colored calla (Zantedeschia spp.). Proc. Kor. J. Hortic. Sci. Technol. 19:98. (in Korean)
5 Kim, H.G. 2014. Chlorophyll fluorescence, graft-take and growth characteristics of cucumber (Cucumis sativus L.) grafted seedlings as affected by light quality and light intensity of LED lamps. MS Thesis. Chonbuk National University. (in Korean)
6 Kim, Y.H. 2000. Design of a prototype system for graft-taking enhancement of grafted seedlings using artificial lighting-Effect of air current speed on the distribution of air temperature and relative humidity in a graft-taking enhancement system- J. Kor. Soc. Agric. Mach. 25:213-220. (in Korean)
7 Kim, Y.H. and C.S. Kim. 2000. Three dimensional visualization for vessel structure of grafted seedlings. Proc. Kor. Soc. Agric. Mach. 5:492-498. (in Korean)
8 Kim, Y.H. and Y.S. Park. 2001. Evapotranspiration rate of grafted seedlings affected by relative humidity and photosynthetic photon flux under artificial lighting. J. Kor. Soc. Agric. Mach. 26:379-384. (in Korean)
9 Koscielniak. J, W. Filek, and J. Biesaga-Koscielniak. 2006. The effect of drought stress on chlorophyll fluorescence in Lolium-Festuca hybrids. Acta Physiol. Plant. 28:149-158. doi:10.1007/s11738-006-0041-y   DOI
10 Kooten, O. and J.F. Snel. 1990. The use of chlorophyll fluorescence nomenclature in plant stress physiology. Photosynth Res. 25:147-50. doi:10.1007/BF00033156   DOI
11 Krause G.H. and F. Weis. 1991. Chlorophyll Fluorescence and Photosynthesis: The Basics. Ann. Rev. Plant Physiol. Plant Mol. Biol. 42:313-349. doi:10.1146/annurev   DOI
12 Kycko, M., B. Zagajewski, S. Lavender, E. Romanowska, and M. Zwijacz-Kozica. 2018. The impact of tourist traffic on the condition and cell structures of Alpine swards. Remote Sens. 10:220-241. doi:10.3390/rs10020220   DOI
13 Lang, M., H.K. Lichtenthaler, M. Sowinska, F. Heisel, and J.A. Miehe. 1996. Fluorescence imaging of water and temperature stress in plant leaves. J. Plant Physiol. 148:613-621. doi:10.1016/S0176-1617(96)80082-4   DOI
14 Schwarz, D., Y. Rouphaelb, G. Collac, and J.H. Venemad. 2010. Grafting as a tool to improve tolerance of vegetables to abiotic stresses. Scientia Horticulturae 127:162-171. doi:10.1016/j.scienta.2010.09.016   DOI
15 Lee, J.M., C. Kubota, S.J. Tsao, Z. Bie, P.H. Echevarria, L. Morra, and M. Oda. 2010. Current status of vegetable grafting diffusion, grafting techniques, automation. Sci. Hortic. 127:93-105. doi:10.1016/j.scienta.2010.08.003   DOI
16 Maxwell, K. and G.N. Johnson. 2000. Chlorophyll fluorescence - a practical guide. J. Exp. Bot. 51:659-668. doi:10.1093/jexbot/51.345.659   DOI
17 Moradi, F. and A.M. Ismail. 2007. Responses of photosynthesis, chlorophyll fluorescence and ROS-scavenging systems to salt stress during seedling and reproductive stages in rice. Ann. Bot. 99:1161-1173. doi:10.1093/aob/mcm052   DOI
18 Baker, N.R. and E. Rosenqvist. 2004. Applications of chlorophyll fluorescence can improve crop production strategies: an examination of future possibilities. J. Exp. Bot. 55:1607-1621. doi:10.1093/jxb/erh196   DOI
19 Willits, D.H. and M.M. Peet. 1999. Using chlorophyll fluorescence to model leaf photosynthesis in greenhouse pepper and tomato. Acta. Hort. 507:311-315. doi:10.17660/Acta-Hortic.1999.507.36   DOI
20 Arnon, D.I. 1949. Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta Vulgaris. Plant Physiol. 24:1-15. doi:10.1104/pp.24.1.1   DOI
21 Buttery, B.R. and R.L Buzzell. 1977. The relationship between chlorophyll content and rate of photosynthesis in soybeans. Can. J. Plant Sci. 57: l-5. doi:10.4141/cjps77-001   DOI
22 Han, S.H, D.H. Kim, G.N. Kim, and J.K. Byun. 2011. Changes of leaf characteristics, pigment content and photosynthesis of Forsythia saxatilis under two different light intensities. J. Kor. For. Soc. 100:609-615. (in Korean)
23 Hazrati, S., Z. Tahmasebi-Sarvestani, S.A.M. Modarres-Sanavy, A. Mokhtassi-Bidgoli, S. Nicola. 2016. Effects of water stress and light intensity on chlorophyll fluorescence parameters and pigments of Aloe vera L. Plant Physiol. Biochem. 106:141-148. doi:10.1016/j.plaphy.2016.04.046   DOI