Browse > Article
http://dx.doi.org/10.12791/KSBEC.2018.27.3.222

Changes of Plant Growth, Leaf Morphology and Cell Elongation of Spinacia oleracea Grown under Different Light-Emitting Diodes  

Lee, Myungok (Dept. of Agricultural Science, Korea National Open University)
Park, Sangmin (Dept. of Agricultural Science, Korea National Open University)
Cho, Eunkyung (Dept. of Agricultural Science, Korea National Open University)
An, Jinhee (Dept. of Agricultural Science, Korea National Open University)
Choi, Eunyoung (Dept. of Agricultural Science, Korea National Open University)
Publication Information
Journal of Bio-Environment Control / v.27, no.3, 2018 , pp. 222-230 More about this Journal
Abstract
This study aimed to determine effects of light-emitting diodes on plant growth, leaf morphology and cell elongation of two cultivars ('World-star' and 'Sushiro') of Spinacia oleracea. Plants were grown in a NFT system for 25 days after transplanting (DAT) under the LEDs [White (W), Red and Blue (RB, ratio 2:1), Blue (B), Red (R) LED] under the same light intensity and photoperiod ($130{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$, 12 hours). The 'World-star' variety was significantly higher in shoot fresh and dry weights, leaf number, and leaf area than the 'Sushiro' variety. For the 'World-star' variety, the two treatments of mixed light (RB) and red light (R) showed a 35% higher shoot dry weight than that of blue light (B) and white light (W) at 25 DAT. In the 'Sushiro' variety, mixed light (RB) treatment, which had the highest shoot fresh and dry weights, showed 40% higher than the white light (W) treatment, which had the lowest shoot fresh and dry weights. Both varieties showed leaf epinasty symptom at 21 DAT only in both mixed light (RB) and red light (R), and red light (R) treatment showed significantly higher symptom than mixed light (RB), indicating the leaf epinasty is associated with red light. Microscopic observations of the cell size in the leaf center and edge parts showed that the cell density of leaf edge under the red light (R) was lower than that in leaf center, supporting previous reports that suggest an association of the cell size difference between the leaf center and edge with the leaf epinasty occurrence. Since the blue light (B) plays a role in alleviating the epinasty symptom caused by the red light (R), it seems necessary to identify the appropriate mixing ratio of the two light sources. In addition, the World-star variety seems to be more suitable for the cultivation of plant factory using LED light sources.
Keywords
photomorphogenic response; leaf epinasty index; photosynthesis rate; specific leaf weight;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Seo, T.C., Y.C. Kim, J.W. Lee, H.K. Yun, and K.Y.Kim. 2002. The effect of dissolved oxygen concentration on the growth and nutrient uptake of spinach and lettuce grown hydroponically in summer season. J. Kor. Soc. Hort. Sci. 43(4):421-424 (in Korean).
2 Sullivan, J.A. and X.W. Deng. 2003. From seed to seed: The role of photoreceptors in Arabidopsis development. Dev. Biol. 260:289-297.   DOI
3 Savvides, A., D. Fanourakis, and W. van Leperen. 2012. Coordination of hydraulic and stomatal conductances across light qualities in cucumber leaves. J. Expt. Bot. 63:1135-1143.   DOI
4 Son, K.H., J.H. Park, D. Kim, and M.M. Oh. 2012. Leaf shape, growth, and phytochemicals in two leaf lettuce cultivars grown under monochromatic light-emitting diodes. Kor. J. Hort. Sci. Technol. 30:664-672.
5 Wang, H., M. Gu, J. Cui, K. Shi, T. Zhou, and J. Yu. 2009. Effects of light quality on CO2 assimilation, chlorophyllfluorescence quenching, expression of Calvin cycle genes and carbohydrate accumulation in Cucumis sativus. J. Photochem. Photobiol. B. 96:30-37.   DOI
6 Yun, H.K., I.S. Kim, and K.C. Yoo. 1997. Effect of substrates growth and nutrient absorption character of shanghai pak choi, garland chrysanthemum and spinach in sandponics. Kor. J. Hort. Sci. 15(1):209-210 (in Korean).
7 Fukuda, N., M. Fujita, Y. Ohta, S. Sase, S. Nishimura, and H. Ezura. 2008. Directional blue light irradiation triggers epidermal cell elongation of abaxial side resulting in inhibition of leaf epinasty in geranium under red light condition. Sci. Hortic. 115:176-182.   DOI
8 Barnes, C. and B. Bugbee. 1991. Morphological responses of wheat to changes in phytochrome photoequilibnum. Plant Physiol 97:359-65.   DOI
9 Carvalho, R.F., M. Takaki, and R.A. Azevedo. 2011. Plant pigments: The many face of light perception. Acta Physiol. Plant 33:241-248.   DOI
10 Christopher, P., Volkenburgh, E.V., 1997. Auxin-induced epinasty of tobacco leaf tissues, a nonethylene-mediated response. Plant Physiol. 113, 603-610.   DOI
11 Goins, G.D., N.C. Yorio, M.M. Sanwo, and C.S. Brown. 1997. Photomorphogenesis, photosynthesis, and seed yield of wheat plants grown under red light-emitting diodes (LEDs) with and without supplemental blue lighting. J. Expt. Bot. 48:1407-1413.   DOI
12 Hwang, M.K., C.S. Huh and Y.J. Seo. 2004. Optic Characteristics comparison and analysis of SMD type Y/G/W HB LED. J. Korean Inst. IIIum. Electr. Install. Eng. 18(4): 15-21.
13 Hogewoning, S.W., G. Trouwborst, H. Maljaars, H. Poorter, W. vanLeperen, and J. Harbinson. 2010. Blue light doseresponses of leaf photosynthesis, morphology, and chemical composition of Cucumis sativus grown under different combinations of red and blue light. J. Expt. Bot. 61:3107-3117.   DOI
14 Kim, H.H., G.D. Goins, R.M. Wheeler, and J.C. Sager. 2004. Green-light supplementation for enhanced lettuce growth under red- and blue-light-emitting diodes. HortScience 39:1617-1622.
15 Johkan, M., K. Shoji, F. Goto, S. Hashida, and T. Yoshigara. 2012. Effect of green light wavelength and intensity on photomorphogenesis and photosynthesis in Lactuca sativa. Environ. Expt. Bot. 75:128-133.   DOI
16 Kendrick R.E., G.H.M. Kronenberg. 1994. Photomorphogenesis in plants. 2nd ed. Kjuwer Academic Publishers, Netherlands.
17 Kozuka T., G. Horiguchi, G. Kim, M. Ohgishi, T. Sakai, and H. Tsukaya. 2005. The different growth responses of the Arabidopsis thaliana leaf blade and the petiole during shade avoidance are regulated by photoreceptors and sugar. Plant Cell Physiol., 46: 213-223.   DOI
18 Lee, J.G., S.S. Oh, S.H. Cha, Y.A. Jang, S.Y. Kim, Y.C. Um, and S.R. Cheong. 2010. Effects of red/blue light ratio and short-term light quality conversion on growth and anthocyanin contents of baby leaf lettuce. J. Bio-Environ. Control 19:351-359.
19 MAFRA. 2016. Agriculture, The major statistics in agriculture, food and rural affairs. Sejong, South Korea. p. 28 (in Korean).
20 Park, H., Y.J. Yu, and E.Y. Choi. 2017. Effects of fluorescent light and light-emitting diodes on Leaf morphology, growth and antioxidant capacity of Salvia plebeia. Protec. Hort. Plant Fact. 26:208-214.   DOI