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Effect of High Temperature and High Humidity on Protein Expression and Plasma Membrane $H^{+}ATPase$ of Umbel with Flower of Onion (Allium cepa L.)  

Ku, Yang-Gyu (Department of Plant Biotechnology, Bioenergy Research Institute, College of Agriculture and Life Science, Chonnam National University)
Park, Won (Department of Plant Biotechnology, Bioenergy Research Institute, College of Agriculture and Life Science, Chonnam National University)
Lee, Eul-Tai (Mokpo Sub-Institute, National Institute of Horticultural & Herbal Science, Rural Development Administration)
Kim, Cheol-Woo (Mokpo Sub-Institute, National Institute of Horticultural & Herbal Science, Rural Development Administration)
Oh, Jeong-Min (Department of Plant Biotechnology, Bioenergy Research Institute, College of Agriculture and Life Science, Chonnam National University)
Jang, Young-Seok (Bioenergy Crop Research Center, National Institute of Crop Science, Rural Development Administration)
Kim, Yong-Kwon (National Agricultural Cooperative Federation, Seed Research & Development Center)
Ahn, Sung-Ju (Department of Plant Biotechnology, Bioenergy Research Institute, College of Agriculture and Life Science, Chonnam National University)
Publication Information
Journal of Bio-Environment Control / v.18, no.2, 2009 , pp. 160-165 More about this Journal
Abstract
This study was undertaken the effect of high temperature and high humidity on protein expression and especially plasma membrane (PM) $H^{+}ATPase$ of umbel with flowers of early cultivar 'Shinsunhwang' and intermediate cultivar 'Maebsihwang' of onion (Allium cepa L.). There were no visible any difference on the protein pattern from before flowering stage to full flowering stage of two onion cultivars, however, seed set stages were revealed induced/deduced protein patterns. At day 18, protein expression pattern of the high temperature and high humidity treatments of two cultivars was significantly reduced compared to controls. Furthermore, various protein expression of the high temperature treatment was more reduced compared to high humidity treatment. PM $H^{+}ATPase$ expression of the control plants of two onion cultivars was clearly shown, but was not detectable under high temperature treatment of the two onion cultivars using western blot analysis. PM $H^{+}ATPase$ expression of the high humidity treatment was faintly detected intermediate cultivar 'Maebsihwang', not early cultivar 'Shinsunhwang'. These results indicate that protein expression pattern and PM $H^{+}ATPase$ under high temperature treatment was considered to be more damaged compared to high humidity.
Keywords
protein expression; PM $H^{+}ATPase$; umbel of onion; seed production; stress condition;
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